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Literature summary for 3.2.1.73 extracted from
- Characterization of a bifunctional xylanase/endoglucanase from yak rumen microorganisms (2011), Appl. Microbiol. Biotechnol., 90, 1933-1942.
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | RuCelA can produce xylo-oligosaccharides and cell-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefor, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial application | uncultured microorganism |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21/pET21a | uncultured microorganism |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethylcellulose | Km-value: 2.71 g/l at 50°C, pH5.0 | uncultured microorganism |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, activity increased to 154% | uncultured microorganism |  |
| Cu2+ | 10 mM, activity decreased to 86% | uncultured microorganism | |
| Mg2+ | 10 mM, activity decreased to 85% | uncultured microorganism | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60600 | - |
calculated mass | uncultured microorganism |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured microorganism | E2DQY5 | RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin | uncultured microorganism |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured microorganism | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 54.3 | - |
carboxymethylcellulose as substrate, pH 5.0, 50°C | uncultured microorganism |
| 58.6 | - |
hydroxyethylcellulose as substrate, pH 5.0, 50°C | uncultured microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
uncultured microorganism | ? | - |
? | |
| cellulose + H2O | filter paper | uncultured microorganism | ? | main products are cellobiose and cellotriose | ? | |
| hydroxyethylcellulose + H2O | - |
uncultured microorganism | ? | - |
? | |
| additional information | no activitiy using beta-1,3-glucan, laminarin, or Avicel as substrates | uncultured microorganism | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bifunctional xylanase/endoglucanase | RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73 | uncultured microorganism |
| endoglucanase | - |
uncultured microorganism |
| RuCelA | - |
uncultured microorganism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
uncultured microorganism |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
uncultured microorganism |
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Release 2023.1 (January 2023)
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