Application | Comment | Organism |
---|---|---|
biofuel production | RuCelA can produce xylo-oligosaccharides and cell-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefor, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial application | uncultured microorganism |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21/pET21a | uncultured microorganism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | Km-value: 2.71 g/l at 50°C, pH5.0 | uncultured microorganism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, activity increased to 154% | uncultured microorganism | |
Cu2+ | 10 mM, activity decreased to 86% | uncultured microorganism | |
Mg2+ | 10 mM, activity decreased to 85% | uncultured microorganism |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60600 | - |
calculated mass | uncultured microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured microorganism | E2DQY5 | RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
Purification (Comment) | Organism |
---|---|
the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin | uncultured microorganism |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured microorganism | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.3 | - |
carboxymethylcellulose as substrate, pH 5.0, 50°C | uncultured microorganism |
58.6 | - |
hydroxyethylcellulose as substrate, pH 5.0, 50°C | uncultured microorganism |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
uncultured microorganism | ? | - |
? | |
cellulose + H2O | filter paper | uncultured microorganism | ? | main products are cellobiose and cellotriose | ? | |
hydroxyethylcellulose + H2O | - |
uncultured microorganism | ? | - |
? | |
additional information | no activitiy using beta-1,3-glucan, laminarin, or Avicel as substrates | uncultured microorganism | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional xylanase/endoglucanase | RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73 | uncultured microorganism |
endoglucanase | - |
uncultured microorganism |
RuCelA | - |
uncultured microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
uncultured microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
uncultured microorganism |