Cloned (Comment) | Organism |
---|---|
expression as GST-fusion protein | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the truncated gene product, devoid of cellulose-binding domain, shows 60% of activity and binds to avicel | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 5 mM, 58% of initial activity | Bacillus subtilis | |
additional information | not inhibitory: EDTA | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 160% of initial activity | Bacillus subtilis | |
Mn2+ | 5 mM, 152% of initial activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis A8-8 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | 1% of the activity with carboxymethyl-cellulose | Bacillus subtilis | ? | - |
? | |
avicel + H2O | 50% of the activity with carboxymethyl-cellulose | Bacillus subtilis | ? | - |
? | |
avicel + H2O | 50% of the activity with carboxymethyl-cellulose | Bacillus subtilis A8-8 | ? | - |
? | |
carboxymethyl-cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
laminarin + H2O | 70% of the activity with carboxymethyl-cellulose | Bacillus subtilis | ? | - |
? | |
laminarin + H2O | 70% of the activity with carboxymethyl-cellulose | Bacillus subtilis A8-8 | ? | - |
? | |
lichenan + H2O | 500% of the activity with carboxymethyl-cellulose | Bacillus subtilis | ? | - |
? | |
lichenan + H2O | 500% of the activity with carboxymethyl-cellulose | Bacillus subtilis A8-8 | ? | - |
? | |
additional information | no substrate: cellobiose | Bacillus subtilis | ? | - |
? | |
additional information | no substrate: cellobiose | Bacillus subtilis A8-8 | ? | - |
? | |
xylan + H2O | 500% of the activity with carboxymethyl-cellulose | Bacillus subtilis | ? | - |
? | |
xylan + H2O | 500% of the activity with carboxymethyl-cellulose | Bacillus subtilis A8-8 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | stable within | Bacillus subtilis |