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Literature summary for 3.2.1.73 extracted from

  • Hong, M.R.; Kim, Y.S.; Joo, A.R.; Lee, J.K.; Kim, Y.S.; Oh, D.K.
    Purification and characterization of a thermostable beta-1,3-1,4-glucanase from Laetiporus sulphureus var. miniatus (2009), J. Microbiol. Biotechnol., 19, 818-822.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 8% inhibition at 1 mM Laetiporus sulphureus
Cu2+ 14% inhibition at 1 mM Laetiporus sulphureus
EDTA 12% inhibition at 1 mM Laetiporus sulphureus
Mg2+ 8% inhibition at 1 mM Laetiporus sulphureus
Mn2+ 67% inhibition at 1 mM Laetiporus sulphureus
additional information no inhibition by Ca2+ and Fe2+ at 1 mM Laetiporus sulphureus
Zn2+ 8% inhibition at 1 mM Laetiporus sulphureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Laetiporus sulphureus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 9% activation at 1 mM Laetiporus sulphureus
Ni2+ 19% activation at 1 mM Laetiporus sulphureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
2 * 26000, SDS-PAGE Laetiporus sulphureus
52000
-
gel filtration Laetiporus sulphureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,3-1,4-glucan + H2O Laetiporus sulphureus from Hordeum vulgare ?
-
?

Organism

Organism UniProt Comment Textmining
Laetiporus sulphureus
-
var. miniatus
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by 16fold ammonium sulfate fractionation, combined cation and anion exchange chromatography, and another anion exchange chromatography Laetiporus sulphureus

Source Tissue

Source Tissue Comment Organism Textmining
additional information the optimum temperature for the fungal growth is 28°C, the fungus does not grow above 42°C Laetiporus sulphureus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29
-
purified native enzyme, substrate is Hordeum vulgare beta-1,3-1,4-glucan Laetiporus sulphureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-1,4-glucan + H2O from Hordeum vulgare Laetiporus sulphureus ?
-
?
beta-1,3-1,4-glucan + H2O from Hordeum vulgare, best substrate Laetiporus sulphureus ?
-
?
carboxymethylcellulose + H2O 18% of the activity with beta-1,3-1,4-glucan from Hordeum vulgare Laetiporus sulphureus ?
-
?
cellulose + H2O 7% of the activity with beta-1,3-1,4-glucan from Hordeum vulgare Laetiporus sulphureus ?
-
?
lichenan + H2O 85% of the activity with beta-1,3-1,4-glucan from Hordeum vulgare Laetiporus sulphureus ?
-
?
additional information also active with laminarin, poor activity with Avicel, rice straw, and xylan, no activity with 4-nitrophenyl-beta-D-hexoside derivatives, substrate specificity, overview Laetiporus sulphureus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Laetiporus sulphureus

Synonyms

Synonyms Comment Organism
beta-1,3-1,4-glucanase
-
Laetiporus sulphureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Laetiporus sulphureus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 90 at temperatures of 60°C and 85°C, the enzyme activity is 85% and 80% of maximal activity, respectively Laetiporus sulphureus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal inactivation follow first-order kinetics, overview Laetiporus sulphureus
65
-
purified native enzyme, half-life is over 68 days Laetiporus sulphureus
70
-
purified native enzyme, half-life is 125 h Laetiporus sulphureus
75
-
purified native enzyme, half-life is 22 h Laetiporus sulphureus
80
-
purified native enzyme, half-life is 1 h Laetiporus sulphureus
85
-
purified native enzyme, half-life is about 5 min Laetiporus sulphureus
90
-
purified native enzyme, half-life is 80 s Laetiporus sulphureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3
-
carboxymethylcellulose pH 4.0, 75°C, substrate from Hordeum vulgare Laetiporus sulphureus
13.5
-
beta-1,3-1,4-glucan pH 4.0, 75°C, substrate from Hordeum vulgare Laetiporus sulphureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Laetiporus sulphureus

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5 79% and 73% of the maximum activity, respectively, at pH 3.5 and pH 4.5 Laetiporus sulphureus