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Literature summary for 3.2.1.73 extracted from
- Structural and catalytic roles of residues located in beta13 strand and the following beta - turn loop in Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (2009), Biochim. Biophys. Acta, 1790, 231-239.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Phe205, Gly207, and Asn208 in the type II turn of the connecting loop may play a role in the catalytic function of beta-glucanase | Fibrobacter succinogenes |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutants cloned into vector pET26b(+) and expressed in Escherichia coli BL21 (DE3) | Fibrobacter succinogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D202L | shows a 1.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| D202N | exhibits a 1.8fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| D206M | shows a 1.1fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| D206N | exhibits a 1.5fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| D206R | exhibits the highest relative activity at 50°C over 10 min, shows a 1.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| F205L | shows a 3.8fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| G201S | shows a 1.5fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| G207N | shows a fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| K200F | is the most heat-sensitive enzyme, retains 72% of activity at 45°C for 10 min, shows a 1.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| K200M | shows a 1.1fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| N208G | shows a fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| R209M | shows a 1.1fold increase, in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| T204F | shows a 2.2fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| W203F | exhibits a 2.4fold increase in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
| W203R | exhibits a 207fold decrease in catalytic efficiency (kcat/KM) compared to the wild-type | Fibrobacter succinogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fibrobacter succinogenes | P17989 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on Ni-NTA affinity column | Fibrobacter succinogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
mutant W203R | Fibrobacter succinogenes |
| 969 | - |
mutant G207- | Fibrobacter succinogenes |
| 1114 | - |
mutant D206R | Fibrobacter succinogenes |
| 1377 | - |
mutant F205L | Fibrobacter succinogenes |
| 1406 | - |
mutant D202L | Fibrobacter succinogenes |
| 1452 | - |
mutant K200M | Fibrobacter succinogenes |
| 1501 | - |
mutant N208G | Fibrobacter succinogenes |
| 1652 | - |
mutant R209M | Fibrobacter succinogenes |
| 1817 | - |
mutant K200F | Fibrobacter succinogenes |
| 2034 | - |
mutant T204F | Fibrobacter succinogenes |
| 2065 | - |
wild-type | Fibrobacter succinogenes |
| 2155 | - |
mutant G201S | Fibrobacter succinogenes |
| 2962 | - |
mutant D206N | Fibrobacter succinogenes |
| 3115 | - |
mutant G207N | Fibrobacter succinogenes |
| 3282 | - |
mutant D206M | Fibrobacter succinogenes |
| 4081 | - |
mutant D202N | Fibrobacter succinogenes |
| 8726 | - |
mutant W203F | Fibrobacter succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lichenan + H2O | - |
Fibrobacter succinogenes | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3-1,4-beta-D-glucanase | - |
Fibrobacter succinogenes |
| beta-glucanase | - |
Fibrobacter succinogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
the wild-type maintains more than 85% of the original activity for 10 min | Fibrobacter succinogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 44 | - |
lichenan | mutant W203R, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 608 | - |
lichenan | mutant G207-, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 699 | - |
lichenan | mutant D206R, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 865 | - |
lichenan | mutant F205L, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 883 | - |
lichenan | mutant D202L, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 911 | - |
lichenan | mutant K200M, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 942 | - |
lichenan | mutant N208G, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1037 | - |
lichenan | mutant R209M, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1141 | - |
lichenan | mutant K200F, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1277 | - |
lichenan | mutant T204F, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1296 | - |
lichenan | wild-type, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1353 | - |
lichenan | mutant G201S, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1860 | - |
lichenan | mutant D206N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 1955 | - |
lichenan | mutant G207N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 2060 | - |
lichenan | mutant D206M, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 2562 | - |
lichenan | mutant D202N, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
| 5476 | - |
lichenan | mutant W203F, calculated as D-glucose units released from lichenan | Fibrobacter succinogenes | |
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