Literature summary for 3.2.1.73 extracted from

Fukamizo, T.; Hayashi, K.; Tamoi, M.; Fujimura, Y.; Kurotaki, H.; Kulminskaya, A.; Kitaoka, M.
Enzymatic hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide substrates (2008), Arch. Biochem. Biophys., 478, 187-194.

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Cloned(Commentary)

Cloned (Comment)	Organism
into pET3a vector and expressed in Escherichia coli strain BL21(DE3)pLysS	Synechocystis sp. PCC 6803

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	substrate inhibition for 4-methylumbelliferyl laminaribiose, but not for 4-methylumbelliferyl cellobiose	Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.058	-	4-methylumbelliferyl cellobiose	-	Synechocystis sp. PCC 6803
0.19	-	4-methylumbelliferyl laminaribiose	-	Synechocystis sp. PCC 6803

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration and ultrafiltration	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl cellobiose + H2O	-	Synechocystis sp. PCC 6803	?	-	?
4-methylumbelliferyl laminaribiose + H2O	-	Synechocystis sp. PCC 6803	?	-	?
barley beta-glucan + H2O	-	Synechocystis sp. PCC 6803	?	-	?
Glcbeta(1-3)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O	-	Synechocystis sp. PCC 6803	glucose + Glcbeta(1-3)Glcbeta(1-4)Glcbeta(1-4)Glc	-	?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O	-	Synechocystis sp. PCC 6803	?	-	?
lichenan + H2O	-	Synechocystis sp. PCC 6803	?	-	?
additional information	does not hydrolyze laminaripentaose and cellopentaose	Synechocystis sp. PCC 6803	?	-	?

Synonyms

Synonyms	Comment	Organism
1,3-1,4-beta-glucanase	-	Synechocystis sp. PCC 6803

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.327	-	4-methylumbelliferyl laminaribiose	-	Synechocystis sp. PCC 6803
1.35	-	4-methylumbelliferyl cellobiose	-	Synechocystis sp. PCC 6803
3.71	-	lichenan	calculated as D-glucose units released from lichenan	Synechocystis sp. PCC 6803
8.4	-	Barley beta-glucan	-	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)