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Literature summary for 3.2.1.73 extracted from

  • Chen, H.; Yan, X.; Liu, X.; Wang, M.; Huang, H.; Jia, X.; Wang, J.
    Purification and characterization of novel bifunctional xylanase, XynIII, isolated from Aspergillus niger A-25 (2006), J. Microbiol. Biotechnol., 16, 1132-1138.
No PubMed abstract available
Search for more literature for 3.2.1.73

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 90.98% residual activity at 1 mM Aspergillus niger
Co2+ 82.19% residual activity at 1 mM Aspergillus niger
Cu2+ 44% residual activity at 1 mM Aspergillus niger
EDTA 79.64% residual activity at 1 mM Aspergillus niger
Hg2+ complete inhibition at 1 mM Aspergillus niger
K+ 95.61% residual activity at 1 mM Aspergillus niger
Mn2+ 37.29% residual activity at 1 mM Aspergillus niger
Pb2+ 79.29% residual activity at 1 mM Aspergillus niger
Zn2+ 88.66% residual activity at 1 mM Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 20.8% increase of activity at 1 mM Aspergillus niger
Mg2+ 5.3% increase of activity at 1 mM Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27900
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger A-25
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose CL-6B column chromatography Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenin + H2O
-
 Aspergillus niger ?
-
 ?
lichenin + H2O
-
 Aspergillus niger A-25 ?
-
 ?
additional information does not hydrolyze carboxymethyl-cellulose or avicel cellulose Aspergillus niger ?
-
 ?
additional information does not hydrolyze carboxymethyl-cellulose or avicel cellulose Aspergillus niger A-25 ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-(1,3-1,4)-glucanase
-
 Aspergillus niger
GluIII
-
 Aspergillus niger
XynIII bifunctional xylanase showing also beta-(1,3-1,4)-glucanase activity Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 sharp decrease in activity up to 60°C, but very stable below 50°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6 5.6
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.6 10.6
-
 Aspergillus niger