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Literature summary for 3.2.1.73 extracted from

  • Wang, J.; Ruan, H.; Zhang, H.; Zhang, Q.; Zhang, H.; He, G.; Shen, S.
    Characterization of a thermostable and acidic-tolerable beta-glucanase from aerobic fungi Trichoderma koningii ZJU-T (2007), J. Food Sci., 72, C452-C456.
    View publication on PubMed
Search for more literature for 3.2.1.73

General Stability

General Stability Organism
in strong acid solution (HCl), beta-glucanase loses activity under conditions of pH 2.0, 1.0, and 0 indicating no tolerance to HCl Trichoderma koningii

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol inhibits activity at a concentration of 5% Trichoderma koningii
Ca2+ 78.65% residual activity at 1 mM Trichoderma koningii
Cu2+ 91.01% residual activity at 0.5 mM Trichoderma koningii
EDTA 47.3% residual activity at 60 mM Trichoderma koningii
Fe2+ 84.27% residual activity at 1 mM Trichoderma koningii
Fe3+ 93.26% residual activity at 1 mM Trichoderma koningii
Li+ 66.29% residual activity at 0.5 mM Trichoderma koningii
Mg2+ 78.65% residual activity at 0.5 mM Trichoderma koningii
Mn2+ 77.53% residual activity at 1 mM Trichoderma koningii
Pb2+ 97.75% residual activity at 0.5 mM Trichoderma koningii
SDS inhibits activity at a concentration of 5% Trichoderma koningii
Urea 14.5% residual activity at 7 mM Trichoderma koningii
Zn2+ 69.66% residual activity at 0.5 mM Trichoderma koningii

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 110.11% activity at 1 mM Trichoderma koningii
K+ 115.73% activity at 1 mM Trichoderma koningii
Li+ 103.25% activity at 1 mM Trichoderma koningii

Organism

Organism UniProt Comment Textmining
Trichoderma koningii
-
-
-
Trichoderma koningii ZJU-T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-100 gel filtration Trichoderma koningii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Trichoderma koningii ?
-
 ?
barley beta-glucan + H2O
-
 Trichoderma koningii ZJU-T ?
-
 ?
carboxymethyl-cellulose + H2O
-
 Trichoderma koningii ?
-
 ?
carboxymethyl-cellulose + H2O
-
 Trichoderma koningii ZJU-T ?
-
 ?
lichenan + H2O
-
 Trichoderma koningii reducing sugar + ?
-
 ?
lichenan + H2O
-
 Trichoderma koningii ZJU-T reducing sugar + ?
-
 ?
additional information the purified enzyme fraction shows no activity on laminarin and xylan Trichoderma koningii ?
-
 ?
additional information the purified enzyme fraction shows no activity on laminarin and xylan Trichoderma koningii ZJU-T ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-glucanase
-
 Trichoderma koningii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Trichoderma koningii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100
-
 Trichoderma koningii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 optimum condition for the beta-glucanase stability Trichoderma koningii
100
-
 even when subjected to 100°C for 3 h, beta-glucanase activity does not show significant reduction Trichoderma koningii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
-
 Trichoderma koningii

pH Range

pH Minimum pH Maximum Comment Organism
2 8
-
 Trichoderma koningii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 optimum condition for the beta-glucanase stability Trichoderma koningii