Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 0.1% | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM | Bacillus licheniformis | |
additional information | no significant effects are found for Ca2+, Mg2+, glycerol, D-sorbitol, boric acid, and EDTA | Bacillus licheniformis | |
Zn2+ | 10 mM | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates at 10 mM | Bacillus licheniformis | |
Fe2+ | activates at 10 mM | Bacillus licheniformis | |
Mn2+ | activates at 10 mM | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus licheniformis EGW039 / CGMCC 0635 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1280 | - |
recombinant enzyme, using barley beta-glucan as a substrate, at 50°C and pH 6.0 | Bacillus licheniformis |
1335 | - |
recombinant enzyme, using lichenan as a substrate, at 50°C and pH 6.0 | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
barley beta-glucan + H2O | - |
Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
lichenan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
lichenan + H2O | - |
Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? | |
additional information | no activity on cellulose | Bacillus licheniformis | ? | - |
? | |
additional information | no activity on cellulose | Bacillus licheniformis EGW039 / CGMCC 0635 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | after incubation at 50, 60, and 70°C for 30 min, the residual activity is 95, 60, and 0%, respectively | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
retains approximately 80% of original activity after treatment at pH 3.0 for 12 h | Bacillus licheniformis |
4 | 6 | the recombinant enzyme is stable between pH 4.0 and 6.0, having 85% of the original activity | Bacillus licheniformis |
8 | - |
retains approximately 80% of original activity after treatment at pH 8.0 for 12 h | Bacillus licheniformis |