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Literature summary for 3.2.1.73 extracted from

  • Cho, K.M.; Hong, S.Y.; Lee, S.M.; Kim, Y.H.; Kahng, G.G.; Kim, H.; Yun, H.D.
    A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains (2006), Appl. Microbiol. Biotechnol., 73, 618-630.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D100A 36% relative activity compared to the wild type enzyme Paenibacillus polymyxa
D106A 34% relative activity compared to the wild type enzyme Paenibacillus polymyxa
D195A 36% relative activity compared to the wild type enzyme Paenibacillus polymyxa
D220A 32% relative activity compared to the wild type enzyme Paenibacillus polymyxa
D258A 35% relative activity compared to the wild type enzyme Paenibacillus polymyxa
D314A 99% relative activity compared to the wild type enzyme Paenibacillus polymyxa
D317A 31% relative activity compared to the wild type enzyme Paenibacillus polymyxa
E196A 39% relative activity compared to the wild type enzyme Paenibacillus polymyxa
E222A no activity Paenibacillus polymyxa
E242A 32% relative activity compared to the wild type enzyme Paenibacillus polymyxa
E262A 36% relative activity compared to the wild type enzyme Paenibacillus polymyxa
E323A 32% relative activity compared to the wild type enzyme Paenibacillus polymyxa
E91A no activity Paenibacillus polymyxa

Organism

Organism UniProt Comment Textmining
Paenibacillus polymyxa
-
-
-
Paenibacillus polymyxa GS01
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
234
-
mutant enzyme D317A, at 50°C Paenibacillus polymyxa
240
-
mutant enzyme D220A, at 50°C Paenibacillus polymyxa
241
-
mutant enzyme E323A, at 50°C Paenibacillus polymyxa
245
-
mutant enzyme E242A, at 50°C Paenibacillus polymyxa
258
-
mutant enzyme D106A, at 50°C Paenibacillus polymyxa
266
-
mutant enzyme D258A, at 50°C Paenibacillus polymyxa
271
-
mutant enzyme E262A, at 50°C Paenibacillus polymyxa
273
-
mutant enzyme D100A, at 50°C Paenibacillus polymyxa
274
-
mutant enzyme D195A, at 50°C Paenibacillus polymyxa
296
-
mutant enzyme D196A, at 50°C Paenibacillus polymyxa
748
-
mutant enzyme D314A, at 50°C Paenibacillus polymyxa
754
-
wild type enzyme, at 50°C Paenibacillus polymyxa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenan + H2O
-
Paenibacillus polymyxa ?
-
?
lichenan + H2O
-
Paenibacillus polymyxa GS01 ?
-
?

Synonyms

Synonyms Comment Organism
Lichenase the multifunctional enzyme domain GH44 possesses cellulase, xylanase, and lichenase activities Paenibacillus polymyxa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Paenibacillus polymyxa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Paenibacillus polymyxa