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Literature summary for 3.2.1.73 extracted from
- A truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase with improved enzymatic activity and thermotolerance (2005), Biochemistry, 44, 9197-9205.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| C-terminally truncated (10 kDa), overexpression in Escherichia coli BL21 (DE3) and Pichia pastoris X-33, mutants with increased catalytic efficiency and thermotolerance | Fibrobacter succinogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | resistent to trypsin protease digestion | Fibrobacter succinogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
lichenan | wild type | Fibrobacter succinogenes |  |
| 2.83 | - |
lichenan | expressed in Pichia pastoris | Fibrobacter succinogenes | |
| 2.89 | - |
lichenan | expressed in Escherichia coli | Fibrobacter succinogenes | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fibrobacter succinogenes | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | expressed in Pichia pastoris | Fibrobacter succinogenes |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose FF column twice, Ni-NTA affinity column for the Escherichia coli protein, Q-Sepharose FF column for the Pichia pastoris protein | Fibrobacter succinogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2065 | - |
wild type, sodium citrate buffer, pH 6.0, 50°C, 10 min | Fibrobacter succinogenes |
| 7980 | - |
expressed in Escherichia coli, sodium citrate buffer, pH 6.0, 50°C, 10 min | Fibrobacter succinogenes |
| 10800 | - |
expressed in Pichia pastoris X-33, sodium citrate buffer, pH 6.0, 50°C, 10 min | Fibrobacter succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lichenan + H2O | - |
Fibrobacter succinogenes | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fsbeta-glucanase | - |
Fibrobacter succinogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.0 | Fibrobacter succinogenes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | - |
Fibrobacter succinogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
80% activity after 10 min | Fibrobacter succinogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | recombinant proteins | Fibrobacter succinogenes |
| 6 | 8 | wild type | Fibrobacter succinogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Fibrobacter succinogenes |
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Release 2023.1 (January 2023)
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