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Literature summary for 3.2.1.73 extracted from

  • Faijes, M.; Perez, X.; Perez, O.; Planas, A.
    Glycosynthase activity of Bacillus licheniformis 1,3-1,4-beta-glucanase mutants: specificity, kinetics, and mechanism (2003), Biochemistry, 42, 13304-13318.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E134A mutant devoid of hydrolase activity but efficiently catalyzing transglycosylation Bacillus licheniformis
E134A/E138A no enzymic activity, but reaction occurs in presence of sodium azide, E138 is the general acid-base catalyst Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride pH 7.0, 35°C Bacillus licheniformis
0.34
-
beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride pH 7.0, 35°C Bacillus licheniformis
1.36
-
beta-D-Glc-(1-3)-alpha-D-Glc-fluoride pH 7.0, 35°C Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-cellobioside + beta-D-Glc-(1-3)-alpha-D-Glc-fluoride
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
4-methylumbelliferyl beta-D-cellobioside + beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
4-methylumbelliferyl beta-D-cellobioside + beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-glucopyranoside
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-xylopyranoside
-
Bacillus licheniformis 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Xyl + F-
-
?
alpha-laminaribiosyl fluoride + 4-nitrophenyl-beta-D-Glc-(1-4)-beta-D-Glc
-
Bacillus licheniformis 4-nitrophenyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + 4-nitrophenyl-beta-D-glucopyranoside
-
Bacillus licheniformis 4-nitrophenyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + 4-nitrophenyl-beta-D-xylopyranoside
-
Bacillus licheniformis 4-nitrophenyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Xyl + F-
-
?
alpha-laminaribiosyl fluoride + methyl beta-D-Glc-(1-3)-beta-D-Glc
-
Bacillus licheniformis methyl beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc + F-
-
?
alpha-laminaribiosyl fluoride + methyl beta-D-Glc-(1-4)-beta-D-Glc
-
Bacillus licheniformis methyl beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F-
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
beta-D-Glc-(1-3)-alpha-D-Glc-fluoride pH 7.0, 35°C Bacillus licheniformis
0.91
-
beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride pH 7.0, 35°C Bacillus licheniformis
1.35
-
beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride pH 7.0, 35°C Bacillus licheniformis