Protein Variants | Comment | Organism |
---|---|---|
E134A | mutant devoid of hydrolase activity but efficiently catalyzing transglycosylation | Bacillus licheniformis |
E134A/E138A | no enzymic activity, but reaction occurs in presence of sodium azide, E138 is the general acid-base catalyst | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | - |
Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | pH 7.0, 35°C | Bacillus licheniformis | |
0.34 | - |
beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | pH 7.0, 35°C | Bacillus licheniformis | |
1.36 | - |
beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | pH 7.0, 35°C | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-cellobioside + beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
4-methylumbelliferyl beta-D-cellobioside + beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
4-methylumbelliferyl beta-D-cellobioside + beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-Glc-(1-4)-beta-D-Glc | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-glucopyranoside | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Bacillus licheniformis | 4-methylumbelliferyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Xyl + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-nitrophenyl-beta-D-Glc-(1-4)-beta-D-Glc | - |
Bacillus licheniformis | 4-nitrophenyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-nitrophenyl-beta-D-glucopyranoside | - |
Bacillus licheniformis | 4-nitrophenyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + 4-nitrophenyl-beta-D-xylopyranoside | - |
Bacillus licheniformis | 4-nitrophenyl-beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Xyl + F- | - |
? | |
alpha-laminaribiosyl fluoride + methyl beta-D-Glc-(1-3)-beta-D-Glc | - |
Bacillus licheniformis | methyl beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-beta-D-Glc + F- | - |
? | |
alpha-laminaribiosyl fluoride + methyl beta-D-Glc-(1-4)-beta-D-Glc | - |
Bacillus licheniformis | methyl beta-D-Glc-(1-3)-beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc + F- | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | pH 7.0, 35°C | Bacillus licheniformis | |
0.91 | - |
beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | pH 7.0, 35°C | Bacillus licheniformis | |
1.35 | - |
beta-D-Glc-(1-4)-beta-D-Glc-(1-4)-beta-D-Glc-(1-3)-alpha-D-Glc-fluoride | pH 7.0, 35°C | Bacillus licheniformis |