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Literature summary for 3.2.1.73 extracted from
- Properties of a thermoactive beta-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli (1991), Biochem. Biophys. Res. Commun., 177, 447-452.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the unusually resistance against inactivation by heat, ethanol or ionic detergents makes the enzyme highly suitable for industrial application in the mashing process of beer brewing | Acetivibrio thermocellus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 10 mM, 28% inhibition | Acetivibrio thermocellus |  |
| CaCl2 | 10 mM, 33% inhibition | Acetivibrio thermocellus | |
| dithiothreitol | 1 mM, 21% inhibition | Acetivibrio thermocellus | |
| EDTA | 10 mM, 18% inhibition | Acetivibrio thermocellus | |
| SDS | 100 mM, 28% inhibition | Acetivibrio thermocellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, recombinant enzyme, SDS-PAGE | Acetivibrio thermocellus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | stable up to concentrations up to 10% w/v | Acetivibrio thermocellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucan + H2O | beta-D-glucan from barley | Acetivibrio thermocellus | additional information | - |
? | |
| lichenan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, recombinant enzyme, SDS-PAGE | Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Acetivibrio thermocellus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 90 | 55°C: about 50% of maximal activity, 90°C: about 30% of maximal activity | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 12.5 | half-maximal activity at pH 5.0 and at pH 12.5 | Acetivibrio thermocellus |
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Release 2023.1 (January 2023)
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