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Literature summary for 3.2.1.73 extracted from

  • Chen, H.; Li, X.L.; Ljungdahl, L.G.
    Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin (1997), J. Bacteriol., 179, 6028-6034.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Orpinomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Orpinomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular recombinant enzyme expressed in Escherichia coli Orpinomyces sp.
-
-
periplasmic space
-
 Orpinomyces sp. 42597
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, recombinant enzyme, SDS-PAGE Orpinomyces sp.
27929
-
 x * 27929, calculation from nucleotide sequence Orpinomyces sp.

Organism

Organism UniProt Comment Textmining
Orpinomyces sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification a N-terminal signal peptide of 29 amino residues is cleaved from the enzyme secreted from Orpinomyces whereas 21 amino acid residues of the signal peptide are removed when the enzyme is produced by E. coli Orpinomyces sp.

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Orpinomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium recombinant enzyme expressed in E. coli Orpinomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3659
-
-
 Orpinomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucan + H2O beta-D-glucan from barley Orpinomyces sp. additional information main products are triose and tetraose ?
lichenin + H2O
-
 Orpinomyces sp. additional information main products are triose and tetraose ?
additional information no hydrolysis of xylan Orpinomyces sp. ?
-
 ?
additional information no hydrolysis of laminarin Orpinomyces sp. ?
-
 ?
additional information no hydrolysis of carboxymethylcellulose Orpinomyces sp. ?
-
 ?
additional information no hydrolysis of pustulan Orpinomyces sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, recombinant enzyme, SDS-PAGE Orpinomyces sp.
? x * 27929, calculation from nucleotide sequence Orpinomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Orpinomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 recombinant enzyme, pH 6.0, 24 h, stable Orpinomyces sp.
45
-
 recombinant enzyme, pH 6.0, 24 h, 28% loss of activity Orpinomyces sp.
50
-
 recombinant enzyme, pH 6.0, 24 h, 41% loss of activity Orpinomyces sp.
55
-
 recombinant enzyme, pH 6.0, 24 h, 70% loss of activity Orpinomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6.2
-
 Orpinomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.4 7 about 80% of maximal activity at pH 5.4 and at pH 7.0 Orpinomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.4 9.8 4°C, stable for at least 24 h Orpinomyces sp.