Cloned (Comment) | Organism |
---|---|
gene lnbA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 37% inhibition at 1 mM, 73% at 50 mM | Lactococcus lactis | |
Cu2+ | complete inhibition at 1-50 mM | Lactococcus lactis | |
Fe3+ | 65% inhibition at 1 mM, complete at 50 mM | Lactococcus lactis | |
Mg2+ | slightly inhibitory at 1-50 mM | Lactococcus lactis | |
Mn2+ | 85% inhibition at 1-50 mM | Lactococcus lactis | |
Zn2+ | 42% inhibition at 1 mM, 87% at 50 mM | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.56 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 6.0, 37°C | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slightly activating at 1-50 mM | Lactococcus lactis | |
Na+ | slightly activating at 1-50 mM | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lactococcus lactis | the enzyme shows hydrolytic activity on chito-oligosaccharides but not on colloidal chitin or chitosan, it has both N-acetyl-beta-D-glucosaminidase and N-acetyl-beta-D-galactosaminidase activity, thus indicating that the enzyme is an N-acetyl-beta-D-hexosaminidase | ? | - |
? | |
additional information | Lactococcus lactis IL1403 | the enzyme shows hydrolytic activity on chito-oligosaccharides but not on colloidal chitin or chitosan, it has both N-acetyl-beta-D-glucosaminidase and N-acetyl-beta-D-galactosaminidase activity, thus indicating that the enzyme is an N-acetyl-beta-D-hexosaminidase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q9CFI2 | ssp. lactis, gene lnbA | - |
Lactococcus lactis IL1403 | Q9CFI2 | ssp. lactis, gene lnbA | - |
Purification (Comment) | Organism |
---|---|
gene lnbA, recombinant His6-tagged enzyme about 2fold from Escherichia coli strain BL21(DE3) by ultrafiltration, nickel affinity chromatography, dialysis and ultrafiltration | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.1 | - |
crude recombinant enzyme, pH 6.0, 37°C, substrate 4-nitrophenyl N-acetyl-beta-D-glucosamine | Lactococcus lactis |
37 | - |
purified recombinant enzyme, pH 6.0, 37°C, substrate 4-nitrophenyl N-acetyl-beta-D-glucosamine | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-galactosamine + H2O | - |
Lactococcus lactis | 4-nitrophenol + N-acetyl-beta-galactosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-galactosamine + H2O | - |
Lactococcus lactis IL1403 | 4-nitrophenol + N-acetyl-beta-galactosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Lactococcus lactis | 4-nitrophenol + N-acetyl-beta-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Lactococcus lactis IL1403 | 4-nitrophenol + N-acetyl-beta-glucosamine | - |
? | |
additional information | the enzyme shows hydrolytic activity on chito-oligosaccharides but not on colloidal chitin or chitosan, it has both N-acetyl-beta-D-glucosaminidase and N-acetyl-beta-D-galactosaminidase activity, thus indicating that the enzyme is an N-acetyl-beta-D-hexosaminidase | Lactococcus lactis | ? | - |
? | |
additional information | substrate specificity analysis, no significant activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl 6-O-sulfo-N-acetyl-beta-D-glucosaminide, 2-acetamido-2-deoxy-beta-D-glucopyranosylazide, 2-acetamido-2-deoxy-beta-D-glucopyranourate, colloidal chitin, and chitosan | Lactococcus lactis | ? | - |
? | |
additional information | the enzyme shows hydrolytic activity on chito-oligosaccharides but not on colloidal chitin or chitosan, it has both N-acetyl-beta-D-glucosaminidase and N-acetyl-beta-D-galactosaminidase activity, thus indicating that the enzyme is an N-acetyl-beta-D-hexosaminidase | Lactococcus lactis IL1403 | ? | - |
? | |
additional information | substrate specificity analysis, no significant activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-arabinopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl 6-O-sulfo-N-acetyl-beta-D-glucosaminide, 2-acetamido-2-deoxy-beta-D-glucopyranosylazide, 2-acetamido-2-deoxy-beta-D-glucopyranourate, colloidal chitin, and chitosan | Lactococcus lactis IL1403 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-NAHA | - |
Lactococcus lactis |
lacto-N-biosidase | UniProt | Lactococcus lactis |
lnba | - |
Lactococcus lactis |
N-acetyl-beta-D-hexosaminidase | - |
Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Lactococcus lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | activity range, profile overview | Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified enzyme, pH 6.0, half-life is 53 h | Lactococcus lactis |
50 | - |
purified enzyme, pH 6.0, 30 min, inactivation | Lactococcus lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.7 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 6.0, 37°C | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | activity range, profile overview | Lactococcus lactis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | purified enzyme, 37°C, 30 min: over 80% activity remaining, 24 h: 40% activity remaining at pH 5.5, over 80% at pH 6.0-7.0, 30% at pH 8.0, unstable below pH 5.5, residual activity after 30 min incubation at pH 4.0-5.0 is below 20% | Lactococcus lactis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme may be responsible for degrading chito-oligosaccharides that are produced by the chitinolytic system of Lactobacillus lactis | Lactococcus lactis |