Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme fragments | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme fragments: GH84C catalytic module fragment, and GH84C fragment comprising the catalytic module and the adjacent CBM32, as well as a fragment with the FN3 module, hanging drop vapor diffusion method, 10-25 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with precipitant solution containing 0.1 M Tris-HCl, pH 7.5, 0.1 M MgCl2, 15% w/v PEG 4000, or 0.1 M sodium acetate, pH 3.5, 4% w/v PEG 8000, at 18°C, structure determination and analysis by combination of small angle X-ray scattering and X-ray crystallography at 1.8-3.3 A resolution | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncated enzyme mutants, GH84C fragments comprising the catalytic module only, or additionally the adjacent CBM32 or the FN3 modules | Clostridium perfringens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Clostridium perfringens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q8XL08 | gene nagJ | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme fragments | Clostridium perfringens |
Subunits | Comment | Organism |
---|---|---|
More | GH84C domain structure with the family 84 catalytic module, GH84C, a family 32 carbohydrate binding module,CBM, a cohesin module, Coh, and at the C-terminus of the enzyme a fibronectin type III module, FN3. Three-dimensional structure, domain orientations, analysis and modelling, overview | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-D-N-acetylglucosaminidase | - |
Clostridium perfringens |
GH84C | - |
Clostridium perfringens |
NagJ | - |
Clostridium perfringens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a large, extracellular carbohydrate-active virulence factor involved in host-pathogen interactions, overview. It is a 1001-amino acid multimodular homologue of the Clostridium perfringens mu-toxin | Clostridium perfringens |