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Literature summary for 3.2.1.52 extracted from
- Molecular cloning and expression profile analysis of a novel beta-D-N-acetylhexosaminidase of domestic silkworm (Bombyx mori) (2010), Genes Cells, 15, 525-536.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene and isozyme BmGlcNAcase 1, expression in Trichoplusia ni TN5 cells using the baculovirus tranfection method | Bombyx mori |
| gene and isozyme BmGlcNAcase 2, DNA and amino acid sequence determnination and analysis, genetic structure, phylogenetic analysis, and sequence comparisons, expression in Trichoplusia ni TN5 cells using the baculovirus tranfection method | Bombyx mori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-acetamido-1,2-deoxynojirimycin | - |
Bombyx mori |  |
| additional information | no inhibition by 1-deoxynojirimycin and 1,4-deoxy-1,4-imino-D-arabinitol; no inhibition by 2-acetamide-1,2-deoxynojirymycin, 1-deoxynojirimycin, and 1,4-deoxy-1,4-imino-D-arabinitol | Bombyx mori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61500 | - |
x * 61500, isozyme BmGlcNAcase 2 | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | P49010 | isozyme BmGlcNAcase 1; isozyme BmGlcNAcase 1 | - |
| Bombyx mori | Q3L6N4 | isozyme A or BmGlcNAcase 2; isozyme BmGlcNAcase 2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| BmN cell | - |
Bombyx mori | - |
| embryo | - |
Bombyx mori | - |
| fat body | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| fat body | of fifth instar larvae, low expression level of BmGlcNAcase 2 | Bombyx mori | - |
| head | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| head | of fifth instar larvae, low expression level of BmGlcNAcase 2 | Bombyx mori | - |
| hemolymph | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| larva | BmGlcNAcase 2, first to fourth instar stage, only in some tissue in the fifth instar larvae stage, overview | Bombyx mori | - |
| larva | first to fifth instar stage | Bombyx mori | - |
| malpighian tubule | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| mid intestine | of fifth instar larvae | Bombyx mori | - |
| mid intestine | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| additional information | no expression of BmGlcNAcase 2 in pupa | Bombyx mori | - |
| ovary | of fifth instar larvae | Bombyx mori | - |
| ovary | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| pupa | - |
Bombyx mori | - |
| silk gland | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| skin | of fifth instar larvae, BmGlcNAcase 1 | Bombyx mori | - |
| skin | of fifth instar larvae, low expression level of BmGlcNAcase 2 | Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-galactosamine + H2O | - |
Bombyx mori | 4-nitrophenol + N-acetylgalactosamine | - |
? | |
| 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Bombyx mori | 4-nitrophenol + N-acetylglucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61500, isozyme BmGlcNAcase 2 | Bombyx mori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BmGlcNAcase 1 | - |
Bombyx mori |
| BmGlcNAcase 2 | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Bombyx mori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | the isozyme shows 70% of maximal activity pH 3.5, 30% at pH 7.5 | Bombyx mori |
| 4.5 | 7.5 | 50% of maximal activity at pH 4.5 and pH 7.5, inactive at pH 3.5 | Bombyx mori |
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