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Literature summary for 3.2.1.52 extracted from

  • Yang, Q.; Liu, T.; Liu, F.; Qu, M.; Qian, X.
    A novel beta-N-acetyl-D-hexosaminidase from the insect Ostrinia furnacalis (Guenee) (2008), FEBS J., 275, 5690-5702.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information structural elements of substrates, the 2-acetamido group and the beta-glycoside bond linkage, are essential for its activity. Transcriptional level increases dramatically before the molting stage Ostrinia furnacalis

Application

Application Comment Organism
additional information Hex1 may be mostly involved in insect chitin catabolism and may be a specific target for pesticide development Ostrinia furnacalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
4-nitrophenyl-beta-N-acetyl-D-glucosaminide
-
Ostrinia furnacalis
0.3
-
GlcNAcbeta(1-4)GlcNAc
-
Ostrinia furnacalis
0.43
-
4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
Ostrinia furnacalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65500
-
2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis Ostrinia furnacalis
67000
-
2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis Ostrinia furnacalis
128000
-
gel filtration Ostrinia furnacalis

Organism

Organism UniProt Comment Textmining
Ostrinia furnacalis Q06GJ0
-
-

Purification (Commentary)

Purification (Comment) Organism
1468fold to homogeneity with an activity yield of 20% by four column chromatography steps (anion exchange chromatography and gel filtration) Ostrinia furnacalis

Source Tissue

Source Tissue Comment Organism Textmining
additional information fifth instar larva integument Ostrinia furnacalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
crude extract Ostrinia furnacalis
25
-
1468fold purified enzyme Ostrinia furnacalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
Ostrinia furnacalis 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
Ostrinia furnacalis 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
?
GlcNAcbeta(1-4)GlcNAc + H2O
-
Ostrinia furnacalis ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Ostrinia furnacalis ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Ostrinia furnacalis ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Ostrinia furnacalis ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Ostrinia furnacalis ?
-
?
additional information it can not hydrolyze the complex N-glycan substrate (GlcNAcbeta-1,2Manalpha-1,6)(GlcNAcbeta-1,2Manalpha-1,3)Manbeta-1,4GlcNAcbeta-1,4GlcNAc-PA as well as the long polymer chitin Ostrinia furnacalis ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis Ostrinia furnacalis

Synonyms

Synonyms Comment Organism
beta-N-acetyl-D-hexosaminidase
-
Ostrinia furnacalis
Hex1
-
Ostrinia furnacalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Ostrinia furnacalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable for 30 min Ostrinia furnacalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
708
-
4-nitrophenyl-beta-N-acetyl-D-glucosaminide
-
Ostrinia furnacalis
766
-
4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
Ostrinia furnacalis
934
-
GlcNAcbeta(1-4)GlcNAc
-
Ostrinia furnacalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Ostrinia furnacalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
stable for 30 min Ostrinia furnacalis

pI Value

Organism Comment pI Value Maximum pI Value
Ostrinia furnacalis IEF electrophoresis
-
4.7