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Literature summary for 3.2.1.52 extracted from

  • Marciniak, J.; Zalewska, A.; Popko, J.; Zwierz, K.
    Optimization of an enzymatic method for the determination of lysosomal N-acetyl-beta-D-hexosaminidase and beta-glucuronidase in synovial fluid (2006), Clin. Chem. Lab. Med., 44, 933-937.
    View publication on PubMed

Application

Application Comment Organism
diagnostics a spectrophotometric method for the determination of lysosomally derived N-acetyl-beta-D-hexosaminidase in synovial fluid on a microplate reader is optimized to improve its utility. The assay is sufficiently sensitive for small volumes of synovial fluid, and is useful for the clinical diagnosis of joint diseases Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
synovial fluid
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
-
Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
in a citrate-phosphate buffer Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.1 pH 3.5: about 45% of maximal activity, pH 5.1: about 65% of maximal activity Homo sapiens