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Literature summary for 3.2.1.52 extracted from

  • Tomiya, N.; Narang, S.; Park, J.; Abdul-Rahman, B.; Choi, O.; Singh, S.; Hiratake, J.; Sakata, S.; Betenbaugh, M.J.; Palter, K.B.; Lee, Y.C.
    Purification, characterization, and cloning of a spodoptera frugiperda SF9 beta-N-acetylhexosaminidase that hydrolyzes terminal N-acetylglucosamine on N-glycan core (2006), J. Biol. Chem., 281, 19545-19560.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-1,2-dideoxynojirimycin
-
Spodoptera frugiperda
N-acetyl-D-glucosamine
-
Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
4-nitrophenyl beta-N-acetyl-D-glucosaminide
-
Spodoptera frugiperda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
SDS-PAGE of de-N-glycosylated Sfhex Spodoptera frugiperda
67000
-
SDS-PAGE of purified Sfhex Spodoptera frugiperda
110000
-
gel filtration of Sfhex Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda Q3LS76
-
-

Purification (Commentary)

Purification (Comment) Organism
SDS-PAGE and affinity chromatography Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
SF-9 cell cell culture Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside, desalted enzyme at pH 5.5 and 37°C Spodoptera frugiperda
0.035
-
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside, desalted enzyme at pH 5.5 and 37°C Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
Spodoptera frugiperda 4-methylumbelliferol + N-acetyl-beta-D-galactosamine
-
?
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Spodoptera frugiperda 4-methylumbelliferol + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
Spodoptera frugiperda 4-methylumbelliferone + beta-N-acetyl-D-glucosamine
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-beta-D-glucosamine
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-beta-D-glucosamine
-
?
GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda ?
-
?
Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
?
p-nitrophenyl-N-acetyl-glucosamine + H2O
-
Spodoptera frugiperda p-nitrophenol + N-acetyl-glucosamine
-
?
[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O
-
Spodoptera frugiperda Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-D-glucosamine
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 61000, N-deglycosylated Sfhex, 2 * 67000, SDS-PAGE Spodoptera frugiperda

Synonyms

Synonyms Comment Organism
Beta-N-acetylhexosaminidase
-
Spodoptera frugiperda
Sfhex
-
Spodoptera frugiperda

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside product MGn-2-aminopyridine Spodoptera frugiperda
0.0007
-
GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
Spodoptera frugiperda
0.0012
-
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
Spodoptera frugiperda
0.0016
-
Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside
-
Spodoptera frugiperda
0.0025
-
GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside product GnM-2-aminopyridine Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Spodoptera frugiperda

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00022
-
2-acetamido-1,2-dideoxynojirimycin
-
Spodoptera frugiperda
2
-
N-acetyl-D-glucosamine
-
Spodoptera frugiperda