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Literature summary for 3.2.1.52 extracted from

  • Mayer, C.; Vocadlo, D.J.; Mah, M.; Rupitz, K.; Stoll, D.; Warren, R.A.; Withers, S.G.
    Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi (2006), FEBS J., 273, 2929-2941.
    View publication on PubMed
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
N-acetyl-glucosamine 0.05% (w/v) Cellulomonas fimi
N-acetylglucosamine 0.05% (w/v) Cellulomonas fimi

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cellulomonas fimi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi
0.053
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 4-nitrophenyl-beta-N-acetyl-D-glucosaminide Cellulomonas fimi
0.066
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 4-nitrophenyl-beta-N-acetyl-D-galactosaminide Cellulomonas fimi

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54190
-
 electrospray mass spectroscopy Cellulomonas fimi
60970
-
 electrospray mass spectroscopy Cellulomonas fimi

Organism

Organism UniProt Comment Textmining
Cellulomonas fimi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-bind resin column chromatography Cellulomonas fimi

Storage Stability

Storage Stability Organism
-20°C, glycerol (20% final concentration) and phosphate buffer at pH 7.3, several months, loss of catalytic activity in diluted buffers within a day at 4 °C Cellulomonas fimi
4°C, elution buffer (20 mM sodium phosphate, 80 mM imidazole pH 7.5 and 300 mM NaCl), several months Cellulomonas fimi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(25R)-3beta-hydroxycholest-5-en-27-oate + H2O
-
 Cellulomonas fimi ?
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Cellulomonas fimi 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Cellulomonas fimi 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Cellulomonas fimi 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?

Synonyms

Synonyms Comment Organism
3-beta-N-acetylglucosaminidase
-
 Cellulomonas fimi
beta-glucosidase
-
 Cellulomonas fimi
beta-N-acetylglucosaminidase
-
 Cellulomonas fimi
Beta-N-acetylhexosaminidase
-
 Cellulomonas fimi
Hex20
-
 Cellulomonas fimi
Nag3
-
 Cellulomonas fimi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi
129
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi
482
-
 4-nitrophenyl-beta-N-acetyl-D-glucosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Cellulomonas fimi
8
-
-
 Cellulomonas fimi

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 inactivation above pH 9.5 Cellulomonas fimi
6.8 8.4
-
 Cellulomonas fimi

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9.5 inactivation above pH 9.5 Cellulomonas fimi
6.8 8.4
-
 Cellulomonas fimi