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Literature summary for 3.2.1.52 extracted from

  • Sanon, A.; Lawrence, F.; Bories, C.; Loiseau, P.M.
    Purification of N-acetyl-beta-D-hexosaminidase from Trichomonas vaginalis (2001), Exp. Parasitol., 97, 169-172.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, about 50% inhibition Trichomonas vaginalis
Cu2+ 1 mM, about 50% inhibition Trichomonas vaginalis
Hg2+ 1 mM, about 50% inhibition Trichomonas vaginalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.648
-
 p-nitrophenyl-N-acetyl-beta-D-glucosaminide
-
 Trichomonas vaginalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Trichomonas vaginalis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
204000
-
 non-denaturing PAGE Trichomonas vaginalis

Organism

Organism UniProt Comment Textmining
Trichomonas vaginalis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichomonas vaginalis

Purification (Commentary)

Purification (Comment) Organism
-
 Trichomonas vaginalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.432
-
-
 Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Trichomonas vaginalis p-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
More SDS-PAGE yields a protein band of about 20000 Da, SDS-PAGE in presence of beta-mercaptoethanol yields protein bands of 64000 Da, 66000 Da and 72000 Da Trichomonas vaginalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 225 min Trichomonas vaginalis
55
-
 40 min, over 70% of the initial activity is retained, half-life: 77 min Trichomonas vaginalis
65
-
 10 min, complete inactivation, half-life: below 3 min Trichomonas vaginalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in 200 mM potassium phosphate buffer Trichomonas vaginalis