Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetyl-beta-D-glucosaminolactone | - |
Sclerotinia fructigena | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | inhibition by excess of substrate | Sclerotinia fructigena |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
N,N-diacetylchitobiose | - |
Sclerotinia fructigena | |
2 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Sclerotinia fructigena |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Sclerotinia fructigena | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
141000 | - |
gel filtration | Sclerotinia fructigena |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Sclerotinia fructigena | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sclerotinia fructigena | - |
- |
- |
Purification (Comment) | Organism |
---|---|
46.7 fold to homogeneity | Sclerotinia fructigena |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Sclerotinia fructigena | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sclerotinia fructigena |
Storage Stability | Organism |
---|---|
0°C, half-life of the purified enzyme is 6 days | Sclerotinia fructigena |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Sclerotinia fructigena | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside | - |
? | |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Sclerotinia fructigena | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
chitin + H2O | after 90 h at 37°C and pH 5.0, poor substrate | Sclerotinia fructigena | N-acetylglucosamine | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Sclerotinia fructigena | N-acetyl-D-glucosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Sclerotinia fructigena | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl galactosaminide + H2O | - |
Sclerotinia fructigena | p-nitrophenol + galactosamine | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Sclerotinia fructigena | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Sclerotinia fructigena | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
crude enzyme, relatively stable | Sclerotinia fructigena |
50 | - |
50% inactivation after 10 min | Sclerotinia fructigena |
60 | - |
complete inactivation after 10 min | Sclerotinia fructigena |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
N-acetylgalactosaminidase activity | Sclerotinia fructigena |
5.4 | - |
N-acetylglucosaminidase activity | Sclerotinia fructigena |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
complete inactivation after 30 min at 25°C | Sclerotinia fructigena |
3 | - |
40% inactivation after 30 min at 25°C | Sclerotinia fructigena |
4 | 11 | stable | Sclerotinia fructigena |