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Literature summary for 3.2.1.52 extracted from

  • Ortiz, J.M.; Gillespie, J.B.; Berkeley, R.C.W.
    An exo-beta-N-acetylglucosaminidase from Bacillus subtilis B. Extraction and purification (1972), Biochim. Biophys. Acta, 289, 174-186.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 M and more cause 100% inhibition Bacillus sp. (in: Bacteria)
Mg2+ 0.5 M causes 78% inhibition, 1 M causes 82% inhibition, 2 M and more cause 100% inhibition Bacillus sp. (in: Bacteria)
NH4+ 20% inhibition with 2 M, 5% inhibition with 2 M Bacillus sp. (in: Bacteria)
Urea 19%, 33% and 40% inhibition with 4, 6 and 8 M Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Bacillus sp. (in: Bacteria)
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 B. subtilis
-

Purification (Commentary)

Purification (Comment) Organism
7000fold to homogeneity Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.3
-
 substrate: p-nirophenyl-2-acetamido-2-deoxyglucose Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
-20°C, crude enzyme, stable Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O
-
 Bacillus sp. (in: Bacteria) N-acetyl-D-hexosamines
-
 ?