Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 and 20 mM causes 10% stimulation | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2-acetamido-D-glucono(1-5)lactone | - |
Bacillus sp. (in: Bacteria) | |
Ag+ | 0.01 mM cause 30% inhibition, 2 mM cause 100% inhibition | Bacillus sp. (in: Bacteria) | |
Ca2+ | - |
Bacillus sp. (in: Bacteria) | |
Cd2+ | 2 mM cause 41% inhibition, 10 mM cause 59% inhibition | Bacillus sp. (in: Bacteria) | |
Co2+ | 10 mM cause 37% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | 0.01 mM, 19% inhibition, 2 mM cause 97% inhibition | Bacillus sp. (in: Bacteria) | |
Mg2+ | - |
Bacillus sp. (in: Bacteria) | |
N-acetyl-D-galactosamine | - |
Bacillus sp. (in: Bacteria) | |
N-acetyl-D-glucosamine | - |
Bacillus sp. (in: Bacteria) | |
O-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-3-O-(D-carboxyethyl)-2-deoxy-D-glucose | substrate inhibition above 0.2 mM | Bacillus sp. (in: Bacteria) | |
p-chloromercuribenzoate | 0.1 mM cause 16% inhibition | Bacillus sp. (in: Bacteria) | |
Zn2+ | 2 mM cause 57% inhibition, 10 mM cause 78% inhibition | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
O-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-3-O-(D-carboxyethyl)-2-deoxy-D-glucose | - |
Bacillus sp. (in: Bacteria) | |
0.11 | - |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
Bacillus sp. (in: Bacteria) | |
0.15 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Bacillus sp. (in: Bacteria) | |
0.31 | - |
N,N-diacetylchitobiose | - |
Bacillus sp. (in: Bacteria) | |
0.35 | - |
N,N,N,N,N-pentaacetylchitopentaose | - |
Bacillus sp. (in: Bacteria) | |
0.38 | - |
N,N',N'',N'''-tetraacetylchitotetraose | - |
Bacillus sp. (in: Bacteria) | |
0.39 | - |
N,N',N''-triacetylchitotriose | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
75000 | - |
sucrose density gradient centrifugation, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Bacillus sp. (in: Bacteria) | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
B. subtilis | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O | - |
Bacillus sp. (in: Bacteria) | 4 N-acetyl-D-glucosamine | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetyl-D-glucosamine + N,N',N''-triacetylchitotriose | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetyl-D-glucosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetyl-D-hexosamines | - |
? | |
O-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-3-O-(D-carboxyethyl)-2-deoxy-D-glucose + H2O | bacterial cell wall disaccharide from Micrococcus lysodeikticus | Bacillus sp. (in: Bacteria) | ? | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Bacillus sp. (in: Bacteria) | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
penta-N-acetylchitopentaose + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
tri-N-acetylchitotriose + H2O | - |
Bacillus sp. (in: Bacteria) | N-acetyl-D-glucosamine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
most stable at this pH, stability rapidly decreasing above or below | Bacillus sp. (in: Bacteria) |