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Literature summary for 3.2.1.52 extracted from
- Purification and properties of beta-N-acetylglucosaminidase from Escherichia coli (1976), J. Bacteriol., 125, 324-331.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Glycopeptide | dimeric | Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| enzyme unstable, extensive purification only in presence of 20% glycerol possible | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Escherichia coli | |
| 2.58 | - |
glycopeptide monomer | - |
Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
gel filtration | Escherichia coli |
| 36000 | - |
1 * 36000, SDS-PAGE | Escherichia coli |
| 38000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Escherichia coli | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1094fold to near homogeneity | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 162.3 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in presence of 20% glycerol stable for at least 2 months, even more stable in 50% glycerol | Escherichia coli |
| -70°C, in presence of 20% glycerol stable for at least 3 months, even more stable in 50% glycerol | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide dimer + H2O | - |
Escherichia coli | N-acetylglucosamine + ? | - |
? | |
| glycopeptide monomer + H2O | - |
Escherichia coli | N-acetylglucosamine + ? | - |
? | |
| murein-lipoprotein complex + H2O | - |
Escherichia coli | N-acetylglucosamine + ? | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Escherichia coli | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Escherichia coli | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
crude enzyme preparation | Escherichia coli |
| 7.7 | - |
purified enzyme | Escherichia coli |
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