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Literature summary for 3.2.1.52 extracted from

  • Keyhani, N.O.; Roseman, S.
    The chitin catabolic cascade in the marine bacterium Vibrio furnissii: Molecular cloning, isolation, and characterization of a periplasmic beta-N-acetylhexosaminidase (1996), J. Biol. Chem., 271, 33425-33432.
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
into Escherichia coli Vibrio furnissii

Inhibitors

Inhibitors Comment Organism Structure
(N-acetyl-D-glucosamine)2 non-competitive Vibrio furnissii
(N-acetyl-D-glucosamine)3-6 competitive inhibition Vibrio furnissii
cellobiose
-
 Vibrio furnissii
Hg2+ with 1 mM 50% of activity remains, with 5 mM 10% of activity remains Vibrio furnissii
KCl with 1 mM 80% of activity remains, with 2 mM 50% of activity remains Vibrio furnissii
lactose
-
 Vibrio furnissii
maltose
-
 Vibrio furnissii
additional information
-
 Vibrio furnissii
NaCl with 1 mM 80% of activity remains, with 2 mM 50% of activity remains Vibrio furnissii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Vibrio furnissii
0.33
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Vibrio furnissii
0.78
-
 (N-acetyl-D-glucosamine)4
-
 Vibrio furnissii
0.83
-
 (N-acetyl-D-glucosamine)3
-
 Vibrio furnissii
1.7
-
 (N-acetyl-D-glucosamine)2
-
 Vibrio furnissii
3.5
-
 (N-acetyl-D-glucosamine)6
-
 Vibrio furnissii
3.7
-
 (N-acetyl-D-glucosamine)5
-
 Vibrio furnissii

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Vibrio furnissii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69380
-
 amino acid calculation Vibrio furnissii
70000
-
 SDS-PAGE Vibrio furnissii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Vibrio furnissii
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio furnissii P96155
-
-

Purification (Commentary)

Purification (Comment) Organism
40fold to homogeneity Vibrio furnissii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii

Storage Stability

Storage Stability Organism
-70°C, purified enzyme, stable for at least 6 months Vibrio furnissii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Vibrio furnissii 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
aryl-beta-N-acetylgalactosaminide + H2O
-
 Vibrio furnissii a phenol + beta-N-acetylgalactosamine
-
 ?
aryl-beta-N-acetylglucosaminide + H2O
-
 Vibrio furnissii ? + beta-N-acetyl-D-glucosamine
-
 ?
chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Vibrio furnissii N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Vibrio furnissii ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Vibrio furnissii ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Vibrio furnissii 2 N,N-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Vibrio furnissii N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Vibrio furnissii deacetylated chitooligosaccharides + acetate
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Vibrio furnissii N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Vibrio furnissii 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Vibrio furnissii 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40 at pH 7.5, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 42 stable for 15 min, at pH 7.5 Vibrio furnissii
20
-
 stable for at least 24 h at room temperature Vibrio furnissii
37
-
 stable for at least 24 h Vibrio furnissii
65
-
 complete loss of activity after 15 min at pH 7.5 Vibrio furnissii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 substrate: (N-acetyl-D-glucosamine)2 Vibrio furnissii
7 8 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii
7
-
 substrate: (N-acetyl-D-glucosamine)3-6 Vibrio furnissii

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 full activity at pH 7.5 after preincubation for 15 min in that range, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii
6
-
 50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 no activity, substrate: (N-acetyl-D-glucosamine)2 Vibrio furnissii
8 8.3 50-60% of activity with (N-acetyl-D-glucosamine)3-6 as substrate, no activity with (N-acetyl-D-glucosamine)2 as substrate Vibrio furnissii
9
-
 50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Vibrio furnissii