Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.52 extracted from

  • Li, S.C.; Li, Y.T.
    Studies on the glycosidases of jack bean meal, III. Crystallisation and properties of beta-N-acetylhexosaminidase (1970), J. Biol. Chem., 245, 5153-5160.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Canavalia ensiformis

Inhibitors

Inhibitors Comment Organism Structure
acetamide with 10 mM 70% of activity remains Canavalia ensiformis
Ag+ complete inactivation with 0.1 mM Canavalia ensiformis
Ba2+ with 10 mM 85% of activity remains Canavalia ensiformis
Cu2+ with 10 mM 78% of activity remains Canavalia ensiformis
Fe3+ with 10 mM 5% of activity remains Canavalia ensiformis
Hg2+ complete inactivation with 0.1 mM Canavalia ensiformis
Mg2+ with 10 mM 85% of activity remains Canavalia ensiformis
Mn2+ with 10 mM 82% of activity remains Canavalia ensiformis
N-acetyl-beta-D-glucosaminolactone with 0.002 mM 65% of activity remains Canavalia ensiformis
N-acetyl-D-galactosamine with 10 mM 20% of activity remains Canavalia ensiformis
N-acetyl-D-glucosamine with 10 mM 88% of activity remains Canavalia ensiformis
Sodium acetate with 10 mM 50% of activity remains Canavalia ensiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Canavalia ensiformis
0.64
-
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
Canavalia ensiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Canavalia ensiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Canavalia ensiformis
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Canavalia ensiformis
-
Jack bean
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Canavalia ensiformis

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Canavalia ensiformis

Source Tissue

Source Tissue Comment Organism Textmining
seed meal Canavalia ensiformis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101.9
-
substrate: p-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Canavalia ensiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Canavalia ensiformis 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
?
alpha1-acid glycoprotein + H2O sialic acid- and galactose-free, from human and chimpanzee Canavalia ensiformis ?
-
?
cyclohexyl beta-N-acetylglucosaminide + H2O
-
Canavalia ensiformis cyclohexanol + beta-N-acetyl-D-glucosamine
-
?
ethyl-beta-N-acetyl-glucosaminide + H2O
-
Canavalia ensiformis ethanol + beta-N-acetyl-glucosamine
-
?
globoside + H2O from human kidney Canavalia ensiformis ?
-
?
methyl-beta-N-acetyl-glucosaminide + H2O
-
Canavalia ensiformis methanol + beta-N-acetyl-glucosamine
-
?
additional information
-
Canavalia ensiformis ?
-
?
N,N'-diacetylchitobiose + H2O
-
Canavalia ensiformis N-acetyl-D-glucosamine
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Canavalia ensiformis N-acetyl-D-hexosamines
-
?
ovalbumin + H2O
-
Canavalia ensiformis N-acetylglucosamine + ?
-
?
ovalbumin glycopeptides + H2O
-
Canavalia ensiformis ?
-
?
ovomucoid + H2O
-
Canavalia ensiformis N-acetylglucosamine + ?
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Canavalia ensiformis 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Canavalia ensiformis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
Tay-Sachs ganglioside + H2O asialo derivative Canavalia ensiformis ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
Canavalia ensiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Canavalia ensiformis
5 6 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Canavalia ensiformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
-
Canavalia ensiformis