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Literature summary for 3.2.1.52 extracted from

  • Edwards, R.G.; Thomas, P.; Westwood, J.H.
    The purification and properties of a beta-N-acetylhexosaminidase from Trichomonas foetus (1975), Biochem. J., 151, 145-148.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-2-deoxy-D-galactose
-
 Tritrichomonas suis
2-acetamido-2-deoxy-D-glucose
-
 Tritrichomonas suis
N-acetyl-D-galactosamine competitive inhibitor Tritrichomonas suis
N-acetyl-D-glucosamine competitive inhibitor Tritrichomonas suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Tritrichomonas suis
0.25
-
 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 Tritrichomonas suis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Tritrichomonas suis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Tritrichomonas suis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Tritrichomonas suis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
784fold Tritrichomonas suis

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Tritrichomonas suis
a

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Tritrichomonas suis

Storage Stability

Storage Stability Organism
4°C, purified enzyme stable for few weeks Tritrichomonas suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Tritrichomonas suis 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Tritrichomonas suis N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Tritrichomonas suis 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Tritrichomonas suis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside or -glucosaminide Tritrichomonas suis