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Literature summary for 3.2.1.52 extracted from

  • Mian, N.; Herries, D.G.; Cowen, D.M.; Batte, E.A.
    The multiple forms and kinetic properties of the N-acetyl-beta-D-hexosaminidases from colonic tumours and mucosa of rats treated with 1,2-dimethylhydrazine (1979), Biochem. J., 177, 319-330.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate Rattus norvegicus
glutathione presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate Rattus norvegicus
L-cysteine presence decreases extent of inactivation by Hg2+, Ag2+, thimerosal or p-hydroxymercuribenzoate Rattus norvegicus
p-nitrophenyl-N-acetyl-beta-D-galactosaminide presence at a concentration 10 times that of p-hydroxymercuribenzoate lowers loss of galactosaminidase-activity by 25.5% Rattus norvegicus
p-nitrophenyl-N-acetyl-beta-D-glucosaminide presence at a concentration 50 times that of Hg2+, Ag+, thimerosal or p-hydroxymercuribenzoate reduces loss of glucosaminidase-activity by 20.5% Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ completely inactivates all 3 enzyme forms in colonic mucosa Rattus norvegicus
dithioerythritol concentration of 2.5 mM decreases activity of the 3 enzyme forms in colonic mucosa by 20% at pH 4.2 and by 25% at pH 7.0, similar results with the 2 forms from colon tumors Rattus norvegicus
Hg2+ completely inactivates all 3 enzyme forms in colonic mucosa Rattus norvegicus
mannosamine
-
 Rattus norvegicus
N-acetyl-beta-D-galactosaminolactone
-
 Rattus norvegicus
N-acetyl-beta-D-glucosaminolactone
-
 Rattus norvegicus
N-acetyl-D-galactosamine
-
 Rattus norvegicus
N-acetyl-D-glucosamine
-
 Rattus norvegicus
p-hydroxymercuribenzoate forms A and I retain 50% of glucosaminidase-activity if treated with 0.005 mM at pH 4.2, 5.6 or 7.0 for 15 min, glucosaminidase-activity of form B is stable at pH 4.2 and 7.0 in presence of 0.005 mM and inactivated by less than 5% at pH 5.6, galactosaminidase-activity is decreased by 50% if form A and I was treated with 0.1 mM at pH 4.2, 5.6 or 7.0, galactosaminidase-activity of form B is stable Rattus norvegicus
thimerosal inhibits activity in mucosal and tumor enzymes, 50% of activity remains after 1 h if form A is treated with 0.20 mM, form B with 1.75 mM and form I with 0.65 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside colonic tumor, form B Rattus norvegicus
0.095
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside colonic mucosa, form B Rattus norvegicus
0.2
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside colonic mucosa, form I Rattus norvegicus
0.26
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside colonic tumor, form B Rattus norvegicus
0.31
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside colonic mucosa, form B Rattus norvegicus
0.33
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside colonic mucosa: form A Rattus norvegicus
0.33
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside colonic tumor: form A Rattus norvegicus
0.53
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside colonic mucosa, form I Rattus norvegicus
0.73
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside colonic tumor, form A Rattus norvegicus
0.76
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside colonic mucosa, form A Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Rattus norvegicus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
colonic mucosa: form A 4800fold, form B 5110fold, form I 4100fold, colonic tumors: form A 5090fold, form B 5620fold Rattus norvegicus
N-acetylhexosaminidase: 63fold Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose seperate active sites for glucosaminidase and galactosaminidase activities Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
colonic carcinoma cell
-
 Rattus norvegicus
-
colonic mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.75
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic mucosa, form I Rattus norvegicus
0.88
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic mucosa, form A Rattus norvegicus
0.94
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic mucosa, form B Rattus norvegicus
1.78
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic tumor, form A Rattus norvegicus
1.97
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, colonic tumor, form B Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O
-
 Rattus norvegicus N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Rattus norvegicus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Rattus norvegicus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
phenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Rattus norvegicus phenol + N-acetyl-beta-D-glucosaminide
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 mucosal enzymes, relatively slow inactivation, tumor B-form shows greater stability than mucosal B-form Rattus norvegicus
55
-
 mucosal enzymes, rapid and almost complete inactivation after 5 min Rattus norvegicus
60
-
 mucosal enzymes, rapid and almost complete inactivation after 5 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Rattus norvegicus