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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of beta-N-acetylhexosaminidase from Pycnoporus cinnabarinus (1981), Agric. Biol. Chem., 45, 239-247.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | slight inhibition at 10 mM | Trametes cinnabarina |  |
| N,N,N,N-tetraacetylchitotetraose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside; with 1 mM 45% of N-glucosaminidase- but only 16% of N-galactosaminidase-activity remains | Trametes cinnabarina | |
| N,N,N-triacetylchitotriose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside; with 1 mM 45% of N-glucosaminidase- but only 19% of N-galactosaminidase-activity remains | Trametes cinnabarina | |
| N,N-diacetylchitobiose | inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside stronger than hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside; with 1 mM 54% of N-glucosaminidase- but only 23% of N-galactosaminidase-activity remains | Trametes cinnabarina | |
| N-acetyl-D-glucosamine | with 10 mM 33-35% of activity remains | Trametes cinnabarina | |
| Sodium acetate | inhibits only N-acetylglucosaminidase-activity, concentration of 10 mM causes 30% inhibition | Trametes cinnabarina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Trametes cinnabarina | |
| 0.54 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Trametes cinnabarina | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trametes cinnabarina | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE | Trametes cinnabarina |
| 120000 | - |
gel filtration | Trametes cinnabarina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Trametes cinnabarina | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes cinnabarina | - |
IFO 6139 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1396fold to homogeneity | Trametes cinnabarina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trametes cinnabarina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O | - |
Trametes cinnabarina | 4 N-acetyl-D-glucosamine | - |
? | |
| N,N'-diacetylchitobiose + H2O | - |
Trametes cinnabarina | N-acetyl-D-glucosamine | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Trametes cinnabarina | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Trametes cinnabarina | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Trametes cinnabarina | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| tri-N-acetylchitotriose + H2O | - |
Trametes cinnabarina | N-acetyl-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000, SDS-PAGE | Trametes cinnabarina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
below 45°C stable for 10 min at pH 2.8 | Trametes cinnabarina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Trametes cinnabarina |
| 3.7 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Trametes cinnabarina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 5 | substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Trametes cinnabarina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
activity is lost below | Trametes cinnabarina |
| 2 | 4 | relatively stable, retains 85% of activity after 45 hours at 5°C | Trametes cinnabarina |
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