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Literature summary for 3.2.1.52 extracted from

  • Ogawa, Y.; Nakamura, R.; Sato, Y.
    Purification of beta-N-acetylhexosaminidase from egg white abd the microsomal and lysosomal fractions of hen oviduct (1983), Agric. Biol. Chem., 47, 2085-2089.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Gallus gallus 5764
-
microsome
-
Gallus gallus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
lysosomal enzyme, SDS-PAGE Gallus gallus
68000
-
egg white-enzyme, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Gallus gallus
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
lysosomal enzyme 550fold, microsomal enzyme 756fold, egg white-enzyme 2206fold Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
egg egg white Gallus gallus
-
oviduct
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, microsomal enzyme Gallus gallus
68.4
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, egg white-enzyme Gallus gallus
84.7
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, lysosomal enzyme Gallus gallus

Storage Stability

Storage Stability Organism
lyophilization Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O
-
Gallus gallus N-acetyl-D-hexosamines
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O egg white, lysosomal and microsomal enzyme Gallus gallus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

Subunits

Subunits Comment Organism
dimer 1 * 68000 + 1 * 53000, SDS-PAGE, microsomal enzyme Gallus gallus