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Literature summary for 3.2.1.52 extracted from
- Isozymes of beta-N-acetylhexosaminidase from pea seeds (Pisum sativum L.) (1987), Plant Physiol., 85, 1118-1122.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | blocks inhibition by Hg2+-ions | Pisum sativum |  |
| Ca2+ | beta-NAHA-II | Pisum sativum | |
| cysteine | prevents inhibition by Hg2+-ions | Pisum sativum | |
| Mg2+ | beta-NAHA-II | Pisum sativum | |
| Mn2+ | beta-NAHA-II | Pisum sativum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | all 4 isozymes | Pisum sativum | |
| Fe2+ | 2 mM causes 30-35% loss of activity of beta-NAHA-IA, 10 mM causes 30-35% loss of activity of beta-NAHA-IB | Pisum sativum | |
| Hg2+ | all 4 isozymes, at 1 mM | Pisum sativum | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | only beta-NAHA-IA, above 3.5 mM | Pisum sativum | |
| Zn2+ | all 4 isozymes, at 10 mM inhibition ranges from 20% for beta-NAHA-IC to 45% for beta-NAHA-II | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-IA | Pisum sativum | |
| 0.34 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-IB | Pisum sativum | |
| 0.49 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-IA | Pisum sativum | |
| 0.51 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-IB | Pisum sativum | |
| 0.58 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-II | Pisum sativum | |
| 0.61 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-II | Pisum sativum | |
| 0.76 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | beta-NAHA-IC | Pisum sativum | |
| 1.71 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | beta-NAHA-IC | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| protein body | - |
Pisum sativum | 42735 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
beta-NAHA-II, 1 * 62000 + 1 * 64000 | Pisum sativum |
| 64000 | - |
beta-NAHA-II, 1 * 62000 + 1 * 64000 | Pisum sativum |
| 210000 | - |
gel filtration, all 4 isozymes | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Pisum sativum | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
pea | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Pisum sativum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4 isozymes, beta-NAHA-II was purified 3200fold to apparent homogeneity, beta-NAHA-IA 770fold, beta-NAHA-IB 1750fold, beta-NAHA-IC 500fold | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Pisum sativum | - |
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.08 | - |
beta-NAHA-IC, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
| 3.17 | - |
beta-NAHA-IA, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
| 7.25 | - |
beta-NAHA-IB, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
| 13.4 | - |
beta-NAHA-II, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, lyophilized | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | - |
Pisum sativum | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Pisum sativum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Pisum sativum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | beta-NAHA-II, 1 * 62000 + 1 * 64000 | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | all 4 isozymes, substrates: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside and p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
beta-NAHA-II, no hydrolysis at pH 6.0 and above, beta-NAHA-IA, -IB and IC show 20-25% of maximum activity at pH 6.0 | Pisum sativum |
| 6 | - |
beta-NAHA-II, no hydrolysis above at pH 6.0 and above, beta-NAHA-IA, -IB and IC show 20-25% of maximum activity at pH 6.0 | Pisum sativum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
loss of 25% of acivity if pH is raised to 8.0 | Pisum sativum |
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