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Literature summary for 3.2.1.52 extracted from

  • Barber, M.S.; Ride, J.P.
    Purification and properties of a wheat leaf N-acetyl-beta-D-hexosaminidase (1989), Plant Sci., 60, 163-172.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-2-deoxyglucono-1,4-lactone
-
Triticum aestivum
acetamide inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Triticum aestivum
acetate inhibits hydrolysis of p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Triticum aestivum
Ag+
-
Triticum aestivum
Cu2+
-
Triticum aestivum
Hg2+
-
Triticum aestivum
N-acetyl-D-galactosamine competitive inhibitor Triticum aestivum
N-acetyl-D-glucosamine competitive inhibitor Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Triticum aestivum
0.29
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Triticum aestivum
-
N-acetyl-D-hexosamines
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 stabilizes activity of purified enzyme Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
wheat
-

Purification (Commentary)

Purification (Comment) Organism
2500fold, 5 major isoenzymes Triticum aestivum

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
leaf primary leaves Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Triticum aestivum

Storage Stability

Storage Stability Organism
-20°C, after 6 weeks no significant loss of activity Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N,N',N''-triacetylchitotrioside + H2O poor substrate Triticum aestivum 4-nitrophenol + N,N',N''-triacetylchitotriose
-
?
chitin oligosaccharides + H2O poor substrate Triticum aestivum N-acetyl-D-glucosamine
-
?
chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Triticum aestivum N-acetyl-D-glucosamine
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Triticum aestivum ?
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
Triticum aestivum ?
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
Triticum aestivum 2 N,N-diacetylchitobiose
-
?
N,N',N''-triacetylchitotriose + H2O
-
Triticum aestivum N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
?
N,N'-diacetylchitobiose + H2O
-
Triticum aestivum deacetylated chitooligosaccharides + acetate
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Triticum aestivum N-acetyl-D-hexosamines
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Triticum aestivum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Triticum aestivum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
p-nitrophenyl-N,N'-diacetylchitobioside + H2O poor substrate Triticum aestivum p-nitrophenol + N,N'-diacetylchitobiose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside and (N-acetyl-D-glucosamine)3-6 Triticum aestivum
5
-
substrate: (N-acetyl-D-glucosamine)2 Triticum aestivum
5.2
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Triticum aestivum