Literature summary for 3.2.1.51 extracted from

Lammerts van Bueren, A.; Ardevol, A.; Fayers-Kerr, J.; Luo, B.; Zhang, Y.; Sollogoub, M.; Bleriot, Y.; Rovira, C.; Davies, G.J.
Analysis of the reaction coordinate of alpha-L-fucosidases: a combined structural and quantum mechanical approach (2010), J. Am. Chem. Soc., 132, 1804-1806.

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of mutant D229 soaked with aryl glycoside substrate 4-nitrophenyl alpha-L-fucoside. X-ray data at 1.95 A resolution reveal an unambiguous electron density for the unhydrolysed substrate, which is in the 1C4 conformation. Trapping of fucosyl-enzyme intermediate on the E288Q variant and analysis at 2.1 A. Again, the observed electron density reveals the trapped beta-L-fucosyl enzyme intermediate, here in the 3S1 conformation with the beta-linkage to the nucleophile Asp229	Bacteroides thetaiotaomicron

Crystallization (Comment)

Organism

crystals of mutant D229 soaked with aryl glycoside substrate 4-nitrophenyl alpha-L-fucoside. X-ray data at 1.95 A resolution reveal an unambiguous electron density for the unhydrolysed substrate, which is in the 1C4 conformation. Trapping of fucosyl-enzyme intermediate on the E288Q variant and analysis at 2.1 A. Again, the observed electron density reveals the trapped beta-L-fucosyl enzyme intermediate, here in the 3S1 conformation with the beta-linkage to the nucleophile Asp229

Bacteroides thetaiotaomicron

Protein Variants

Protein Variants	Comment	Organism
D229N	inactive, mutation of the catalytic nucleophile. Crystallization data	Bacteroides thetaiotaomicron
E288Q	acid/base variant, crystallization data in complex with substrate 2-fluoro fucosyl fluoride	Bacteroides thetaiotaomicron

Organism

Organism	UniProt	Comment	Textmining
Bacteroides thetaiotaomicron	-	-	-
Bacteroides thetaiotaomicron Bt2970	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis	Bacteroides thetaiotaomicron	?	-	?
additional information	series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis	Bacteroides thetaiotaomicron Bt2970	?	-	?