Protein Variants | Comment | Organism |
---|---|---|
D124G | kcat/Km at 65°C and pH 6.3 is 3.7fold lower than the wild-type value | Saccharolobus solfataricus |
D146G | kcat/Km at 65°C and pH 6.3 is 1.5fold lower than the wild-type value | Saccharolobus solfataricus |
E292G | kcat/Km at 65°C and pH 6.3 is 310.6fold lower than the wild-type value | Saccharolobus solfataricus |
E292G | kcat/Km at 65°C and pH 6.3 is 310.6fold lower than wild-type value | Saccharolobus solfataricus |
E58G | kcat/Km at 65°C and pH 6.3 is 1897fold lower than the wild-type value | Saccharolobus solfataricus |
E58G | kcat/Km is 10.8-3897fold lower than wild-type value depending on pH-value and buffer | Saccharolobus solfataricus |
H123G | kcat/Km at 65°C and pH 6.3 is 1719.8fold lower than the wild-type value | Saccharolobus solfataricus |
H46G | kcat/Km at 65°C and pH 6.3 is 410fold lower than the wild-type value | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
0.033 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme D146G | Saccharolobus solfataricus | |
0.056 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme E292G | Saccharolobus solfataricus | |
0.088 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme D124G | Saccharolobus solfataricus | |
0.26 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH5.0, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
4.37 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH5.0, 65°C, mutant enzyme H46G | Saccharolobus solfataricus | |
17 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme H46G | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + alpha-L-fucose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.96 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme E292G | Saccharolobus solfataricus | |
224 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme D146G | Saccharolobus solfataricus | |
240 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme D124G | Saccharolobus solfataricus | |
287 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
419 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme E292G | Saccharolobus solfataricus | |
419 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH 6.3, 65°C, mutant enzyme H46G | Saccharolobus solfataricus | |
430 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH5.0, 65°C, wild-type enzyme | Saccharolobus solfataricus | |
495 | - |
4-nitrophenyl-alpha-L-fucopyranoside | pH5.0, 65°C, mutant enzyme H46G | Saccharolobus solfataricus |