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Literature summary for 3.2.1.51 extracted from

  • Carlsen, R.B.; Pierce, J.G.
    Purification and properties of an alpha-L-fucosidase from rat epididymis (1972), J. Biol. Chem., 247, 23-32.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ effective inhibition at 5 mM Rattus sp.
Cd2+ effective inhibition at 5 mM Rattus sp.
Cu2+ effective inhibition at 5 mM Rattus sp.
Hg2+ effective inhibition at 5 mM Rattus sp.
Ni2+ effective inhibition at 5 mM Rattus sp.
Pb2+ effective inhibition at 5 mM Rattus sp.
Zn-acetate more than 90% inhibition at 5 mM Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 60000 + 2 * 2 * 47000, SDS-PAGE Rattus sp.
60000
-
2 * 60000 + 2 * 2 * 47000, SDS-PAGE Rattus sp.
210000 220000 sedimentation equilibrium centrifugation Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
IgG Fc peptide + H2O Rattus sp. human, rabbit, horse alpha-L-fucose + ?
-
?
IgG L peptide + H2O Rattus sp.
-
alpha-L-fucose + ?
-
?
LH-beta peptic glycopeptide + H2O Rattus sp.
-
alpha-L-fucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 0.6% carbohydrate per native molecule Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
near homogeneity Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
epididymis
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.8
-
-
Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
Rattus sp. 4-nitrophenol + alpha-L-fucose
-
?
IgG Fc peptide + H2O from human, rabbit, horse Rattus sp. alpha-L-fucose + ?
-
?
IgG Fc peptide + H2O human, rabbit, horse Rattus sp. alpha-L-fucose + ?
-
?
IgG L peptide + H2O
-
Rattus sp. alpha-L-fucose + ?
-
?
LH-beta peptic glycopeptide + H2O
-
Rattus sp. alpha-L-fucose + ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 60000 + 2 * 2 * 47000, SDS-PAGE Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
Rattus sp.