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Literature summary for 3.2.1.51 extracted from

  • Alhadeff, J.A.; Miller, A.L.; Wenaas, H.; Vedvick, T.; O'Brien, J.S.
    Human liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies (1975), J. Biol. Chem., 250, 7106-7113.
    View publication on PubMed
Search for more literature for 3.2.1.51

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 13% activation at 0.2 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT Homo sapiens
Hg2+ complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 4-methylumbelliferyl-alpha-L-fucopyranoside
-
 Homo sapiens
0.43
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 22% stimulation at 5 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
 sedimentation equilibrium centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Homo sapiens low activity alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Homo sapiens lacto-N-fucopentaose I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Homo sapiens 2'-fucosyllactose alpha-L-fucose + lactose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 1% carbohydrate content per molecule Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2step affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.2
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-L-fucose
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O low activity Homo sapiens alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose I Homo sapiens alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Homo sapiens alpha-L-fucose + lactose
-
 ?

Subunits

Subunits Comment Organism
oligomer subunits of 50.1 kDa each, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 gradual loss of activity after 6 min for the purified enzyme, in crude state: stable for 4 h Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
 one of two pH-optima Homo sapiens
6.5
-
 second pH-optimum Homo sapiens