Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuriphenylsulfonic acid | slight inhibition at 1 mM | Prunus dulcis | |
Ca2+ | alpha-fucosidase I, slight inhibition at 10 mM | Prunus dulcis | |
L-cysteine | alpha-fucosidase I, slight inhibition at 10 mM | Prunus dulcis | |
Mg2+ | slight inhibition at 10 mM | Prunus dulcis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
lacto-N-fucopentaitol III | alpha-fucosidase I | Prunus dulcis | |
0.1 | - |
lacto-N-fucopentaitol II | alpha-fucosidase I | Prunus dulcis | |
0.67 | - |
2'-fucosyllactitol | alpha-fucosidase II | Prunus dulcis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | alpha-fucosidase II, 21% activation at 10 mM | Prunus dulcis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-fucosyllactitol + H2O | Prunus dulcis | alpha-fucosidase II highly active | alpha-L-fucose + lactitol | - |
? | |
3-fucosyllactitol + H2O | Prunus dulcis | low activity | alpha-L-fucose + lactitol | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O | Prunus dulcis | only hydrolysed by alpha-L-fucosidase II | Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O | Prunus dulcis | lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide | Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Prunus dulcis | only hydrolysed by alpha-fucosidase II | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Prunus dulcis | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O | Prunus dulcis | low activity | alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc | - |
? | |
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | Prunus dulcis | low activity for alpha-L-fucosidase II | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | Prunus dulcis | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
lacto-N-fucopentaitol III + H2O | Prunus dulcis | low activity for alpha-L-fucosidase II | alpha-L-fucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus dulcis | - |
almond | - |
Purification (Comment) | Organism |
---|---|
partial, two forms | Prunus dulcis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
emulsin | two forms, alpha-fucosidase I and II | Prunus dulcis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
alpha-L-fucosidase II | Prunus dulcis |
1.46 | - |
alpha-L-fucosidase I | Prunus dulcis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-fucosyllactitol + H2O | alpha-fucosidase II highly active | Prunus dulcis | alpha-L-fucose + lactitol | - |
? | |
3-fucosyllactitol + H2O | low activity | Prunus dulcis | alpha-L-fucose + lactitol | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O | only hydrolysed by alpha-L-fucosidase II | Prunus dulcis | Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide | Prunus dulcis | Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | only hydrolysed by alpha-fucosidase II | Prunus dulcis | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Prunus dulcis | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O | low activity | Prunus dulcis | alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc | - |
? | |
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | low activity for alpha-L-fucosidase II | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
lacto-N-fucopentaitol II + H2O | - |
Prunus dulcis | alpha-L-fucose + ? | - |
? | |
lacto-N-fucopentaitol III + H2O | low activity for alpha-L-fucosidase II | Prunus dulcis | alpha-L-fucose + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | alpha-fucosidase II | Prunus dulcis |
5.5 | - |
alpha-fucosidase I | Prunus dulcis |