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Literature summary for 3.2.1.51 extracted from

  • Ogata-Arakawa, M.; Muramatsu, T.; Kobata, A.
    alpha-L-Fucosidases from almond emulsin: characterization of the two enzymes with different specificities (1977), Arch. Biochem. Biophys., 181, 353-358.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuriphenylsulfonic acid slight inhibition at 1 mM Prunus dulcis
Ca2+ alpha-fucosidase I, slight inhibition at 10 mM Prunus dulcis
L-cysteine alpha-fucosidase I, slight inhibition at 10 mM Prunus dulcis
Mg2+ slight inhibition at 10 mM Prunus dulcis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
lacto-N-fucopentaitol III alpha-fucosidase I Prunus dulcis
0.1
-
lacto-N-fucopentaitol II alpha-fucosidase I Prunus dulcis
0.67
-
2'-fucosyllactitol alpha-fucosidase II Prunus dulcis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ alpha-fucosidase II, 21% activation at 10 mM Prunus dulcis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-fucosyllactitol + H2O Prunus dulcis alpha-fucosidase II highly active alpha-L-fucose + lactitol
-
?
3-fucosyllactitol + H2O Prunus dulcis low activity alpha-L-fucose + lactitol
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O Prunus dulcis only hydrolysed by alpha-L-fucosidase II Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O Prunus dulcis lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Prunus dulcis only hydrolysed by alpha-fucosidase II alpha-L-fucose + lactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Prunus dulcis 2'-fucosyllactose alpha-L-fucose + lactose
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O Prunus dulcis low activity alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O Prunus dulcis low activity for alpha-L-fucosidase II alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O Prunus dulcis lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
lacto-N-fucopentaitol III + H2O Prunus dulcis low activity for alpha-L-fucosidase II alpha-L-fucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Prunus dulcis
-
almond
-

Purification (Commentary)

Purification (Comment) Organism
partial, two forms Prunus dulcis

Source Tissue

Source Tissue Comment Organism Textmining
emulsin two forms, alpha-fucosidase I and II Prunus dulcis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
alpha-L-fucosidase II Prunus dulcis
1.46
-
alpha-L-fucosidase I Prunus dulcis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactitol + H2O alpha-fucosidase II highly active Prunus dulcis alpha-L-fucose + lactitol
-
?
3-fucosyllactitol + H2O low activity Prunus dulcis alpha-L-fucose + lactitol
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O only hydrolysed by alpha-L-fucosidase II Prunus dulcis Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide Prunus dulcis Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O only hydrolysed by alpha-fucosidase II Prunus dulcis alpha-L-fucose + lactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Prunus dulcis alpha-L-fucose + lactose
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O low activity Prunus dulcis alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O low activity for alpha-L-fucosidase II Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
?
lacto-N-fucopentaitol II + H2O
-
Prunus dulcis alpha-L-fucose + ?
-
?
lacto-N-fucopentaitol III + H2O low activity for alpha-L-fucosidase II Prunus dulcis alpha-L-fucose + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.5 alpha-fucosidase II Prunus dulcis
5.5
-
alpha-fucosidase I Prunus dulcis