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Literature summary for 3.2.1.51 extracted from
- Purification and properties of two forms of human alpha-L-fucosidase (1980), Biochim. Biophys. Acta, 614, 476-488.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | - |
Homo sapiens |  |
| 0.125 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | alpha-fucosidase I | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
alpha-fucosidase II, gel filtration | Homo sapiens |
| 100000 | - |
gel filtration, alpha-fucosidase I | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | lacto-N-fucopentaose I | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
| Fuc-alpha-(1-6)-GlcNAc + H2O | Homo sapiens | - |
alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
| lacto-N-fucopentaose II + H2O | Homo sapiens | preferentially hydrolysed by alpha-fucosidase II | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | alpha-fucosidase I: sialoglycoprotein, mannose-rich: alpha-fucosidase II | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, two forms: alpha-fucosidase I and II, affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| urine | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
| Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose I | Homo sapiens | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Homo sapiens | alpha-L-fucose + lactose | - |
? | |
| Fuc-alpha-(1-6)-GlcNAc + H2O | - |
Homo sapiens | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
| lacto-N-fucopentaose II + H2O | preferentially hydrolysed by alpha-fucosidase II | Homo sapiens | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha-fucosidase I, 2 * 50000, SDS-PAGE, can aggregate to tetra- and hexamers | Homo sapiens |
| monomer | alpha-fucosidase II, 1 * 50000, SDS-PAGE, less-biologically active form, can aggregate to tetra- and hexamers | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
alpha-fucosidase I: 50% loss of activity in 40 min, alpha-fucosidase II: 20% loss of activity in 40 min | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
alpha-fucosidase II | Homo sapiens |
| 5.3 | - |
alpha-fucosidase I | Homo sapiens |
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