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Literature summary for 3.2.1.51 extracted from

  • Kobata, A.
    alpha-L-Fucosidases from almond emulsin (1982), Methods Enzymol., 83, 625-631.
    View publication on PubMed
Search for more literature for 3.2.1.51

General Stability

General Stability Organism
60% loss of activity after lyophilization Prunus dulcis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 lacto-N-fucopentaitol III alpha-fucosidase I Prunus dulcis
0.1
-
 lacto-N-fucopentaitol II alpha-fucosidase I Prunus dulcis
0.67
-
 2'-fucosyllactitol alpha-fucosidase II Prunus dulcis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-fucosyllactitol + H2O Prunus dulcis alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages alpha-L-fucose + lactitol
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O Prunus dulcis alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
 ?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O Prunus dulcis lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
lacto-N-fucopentaitol III + H2O Prunus dulcis
-
 alpha-L-fucose + ?
-
 ?
lacto-N-fucopentaose II + H2O Prunus dulcis alpha-fucosidase II alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?

Organism

Organism UniProt Comment Textmining
Prunus dulcis
-
 almond
-

Purification (Commentary)

Purification (Comment) Organism
partial, two forms Prunus dulcis

Source Tissue

Source Tissue Comment Organism Textmining
emulsin two forms, alpha-fucosidase I and II Prunus dulcis
-

Storage Stability

Storage Stability Organism
frozen, more than 90% of its initial activity after 3 months Prunus dulcis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactitol + H2O alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages Prunus dulcis alpha-L-fucose + lactitol
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group Prunus dulcis alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group Prunus dulcis alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
 ?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
lacto-N-fucopentaitol II + H2O
-
 Prunus dulcis alpha-L-fucose + ?
-
 ?
lacto-N-fucopentaitol III + H2O
-
 Prunus dulcis alpha-L-fucose + ?
-
 ?
lacto-N-fucopentaose II + H2O alpha-fucosidase II Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.5 alpha-fucosidase II Prunus dulcis
5.5
-
 alpha-fucosidase I Prunus dulcis