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Literature summary for 3.2.1.51 extracted from
- Purification of Pomacea canaliculata ?-Fucosidase Isoforms with Different Thermostabilities. (1998), Biosci. Biotechnol. Biochem., 62, 807-810.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
4-nitrophenyl-alpha-L-fucopyranoside | isoform F2 | Pomacea canaliculata |  |
| 0.242 | - |
4-nitrophenyl-alpha-L-fucopyranoside | isoform F1 | Pomacea canaliculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | - |
gel filtration | Pomacea canaliculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pomacea canaliculata | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 4.7%, w/w, mannose content for isoform F1, 5%, w/w, mannose content for isoforms F2 | Pomacea canaliculata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques, two isoforms: F1, F2 | Pomacea canaliculata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| viscus | - |
Pomacea canaliculata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.69 | - |
isoform F2 | Pomacea canaliculata |
| 5.89 | - |
isoform F1 | Pomacea canaliculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Pomacea canaliculata | 4-nitrophenol + alpha-L-fucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | contains subunits of 55 kDa and 52 kDa, SDS-PAGE | Pomacea canaliculata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | isoform F2: stable up to | Pomacea canaliculata |
| 65 | - |
isoform F1: stable up to | Pomacea canaliculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.5 | isoforms F1 | Pomacea canaliculata |
| 4 | 6 | isoforms F2 | Pomacea canaliculata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | isoform F1: stable within | Pomacea canaliculata |
| 4.5 | 7 | isoform F2: stable within | Pomacea canaliculata |
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Release 2023.1 (January 2023)
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