Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | 34% inhibition at 0.2%, w/v | Bacillus fulminans | |
ethylenediaminetetraacetic acid | 68% inhibition at 0.1 mM | Bacillus fulminans | |
Hg2+ | marked inhibition at 0.1 mM | Bacillus fulminans | |
Mn2+ | slight inhibition at 0.1 mM | Bacillus fulminans | |
SDS | 42% inhibition at 0.02%, w/v | Bacillus fulminans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
porcine submaxillary mucine, desialyzed | - |
Bacillus fulminans | |
1.6 | - |
glycopeptide from desialyzed porcine submaxillary mucin | - |
Bacillus fulminans | |
2.5 | - |
2'-fucosyllactose | - |
Bacillus fulminans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Bacillus fulminans | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | 80000 | gel filtration | Bacillus fulminans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Bacillus fulminans | lacto-N-fucopentaose I | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Bacillus fulminans | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O | Bacillus fulminans | - |
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O | - |
? | |
glycopeptide + H2O | Bacillus fulminans | desialyzed porcine submaxillary mucin | alpha-L-fucose + ? | - |
? | |
glycopeptide + H2O | Bacillus fulminans | meconium H-1, H-2 and X-1 | alpha-L-fucose + ? | - |
? | |
glycoprotein + H2O | Bacillus fulminans | specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides | alpha-L-fucose + ? | - |
? | |
meconium H-1 + H2O | Bacillus fulminans | - |
alpha-L-fucose + ? | - |
? | |
meconium H-2 + H2O | Bacillus fulminans | - |
alpha-L-fucose + ? | - |
? | |
meconium X-1 + H2O | Bacillus fulminans | low activity | alpha-L-fucose + ? | - |
? | |
submaxillary mucin + H2O | Bacillus fulminans | desialyzed and not, desialyzed mucine best substrate tested | alpha-L-fucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus fulminans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
near homogeneity, chromatography techniques | Bacillus fulminans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Bacillus fulminans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
- |
Bacillus fulminans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose I | Bacillus fulminans | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Bacillus fulminans | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O | - |
Bacillus fulminans | alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O | - |
? | |
glycopeptide + H2O | from meconium H-1, H-2 and X-1 | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
glycopeptide + H2O | desialyzed porcine submaxillry mucin | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
glycopeptide + H2O | desialyzed porcine submaxillary mucin | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
glycopeptide + H2O | meconium H-1, H-2 and X-1 | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
glycopeptide from desialyzed porcine submaxillary mucin + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
glycoprotein + H2O | specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
meconium H-1 + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
meconium H-2 + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
meconium X-1 + H2O | low activity | Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
porcine submaxillary mucine, desialyzed + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
stomach + H2O | - |
Bacillus fulminans | alpha-L-fucose + ? | - |
? | |
submaxillary mucin + H2O | desialyzed and not, desialyzed mucine best substrate tested | Bacillus fulminans | alpha-L-fucose + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
7% loss of activity after 5 min | Bacillus fulminans |
70 | - |
77% loss of activity after 5 min | Bacillus fulminans |