Literature summary for 3.2.1.49 extracted from

Harun-Or-Rashid, M.; Matsuzawa, T.; Satoh, Y.; Shiraishi, T.; Ando, M.; Sadik, G.; Uda, Y.
Purification and characterization of alpha-N-acetylgalactosaminidases I and II from the starfish Asterina amurensis (2010), Biosci. Biotechnol. Biochem., 74, 256-261.

Inhibitors

Inhibitors	Comment	Organism
Ag+	moderate inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II	Asterina amurensis
Ag+	-	Homo sapiens
Ca2+	-	Homo sapiens
Cu2+	-	Homo sapiens
D-galactose	about 10% inhibition of isozymes alpha-GalNAcase I and isozyme alpha-GalNAcase II	Asterina amurensis
D-galactose	-	Homo sapiens
D-glucose	-	Homo sapiens
Hg2+	slight inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II	Asterina amurensis
Hg2+	-	Homo sapiens
K+	-	Homo sapiens
Mg2+	-	Homo sapiens
Mn2+	-	Homo sapiens
additional information	no inhibition by D-glucose, N-acetyl-D-glucosamine, and Mn2+	Asterina amurensis
N-acetyl-D-galactosamine	57% and 62% inhibition of isozyme alpha-GalNAcase I and isozyme alpha-GalNAcase II	Asterina amurensis
N-acetyl-D-galactosamine	-	Homo sapiens
N-acetyl-D-glucosamine	-	Homo sapiens
Na+	-	Homo sapiens
Zn2+	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
1.04	-	GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide	pH 4.2, 37°C, isozyme alpha-GalNAcase I	Asterina amurensis
1.79	-	GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide	pH 4.2, 37°C	Homo sapiens
3.97	-	GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide	pH 4.2, 37°C, isozyme alpha-GalNAcase II	Asterina amurensis

Metals/Ions

Metals/Ions	Comment	Organism
Ca2+	slight activation of both isozymes	Asterina amurensis
Cu2+	slight activation of both isozymes	Asterina amurensis
K+	slight activation of both isozymes	Asterina amurensis
Mg2+	slight activation of both isozymes	Asterina amurensis
Na+	slight activation of both isozymes	Asterina amurensis
Zn2+	slight activation of both isozymes	Asterina amurensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	isozyme alpha-GalNAcase II, gel filtration	Asterina amurensis
43000	-	1 * 43000, isozyme alpha-GalNAcase II, SDS-PAGE	Asterina amurensis
46000	-	x * 46000, native enzyme, SDS-PAGE	Homo sapiens
47000	-	x * 47000, isozyme alpha-GalNAcase I, SDS-PAGE	Asterina amurensis
430000	-	about, isozyme alpha-GalNAcase I, gel filtration	Asterina amurensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins	?	-	?
additional information	Asterina amurensis	the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Asterina amurensis	-	-	-
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from placenta by 4-aminophenyl thio-beta-D-galactose-ceramide hexoside-resin affinity	Homo sapiens
native isozymes alpha-GalNAcase I and II are isolated from liver homogenate by ammonium sulfate fractionation and separated from each other by gel filtration. Then they are further individually purified by cation exchange chromatography and gel filtration, and additionally by hydroxylapatite chromatography for isozyme alpha-GalNAcase II. Isozyme alpha-GalNAcase I is purified 440fold, isozyme alpha-GalNAcase II 544fold	Asterina amurensis

Purification (Comment)

Organism

native enzyme from placenta by 4-aminophenyl thio-beta-D-galactose-ceramide hexoside-resin affinity

Homo sapiens

native isozymes alpha-GalNAcase I and II are isolated from liver homogenate by ammonium sulfate fractionation and separated from each other by gel filtration. Then they are further individually purified by cation exchange chromatography and gel filtration, and additionally by hydroxylapatite chromatography for isozyme alpha-GalNAcase II. Isozyme alpha-GalNAcase I is purified 440fold, isozyme alpha-GalNAcase II 544fold

Asterina amurensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
alimentary canal	-	Asterina amurensis	-
liver	-	Asterina amurensis	-
placenta	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.5	-	purified placental enzyme	Homo sapiens
19.56	-	purified isozyme alpha-GalNAcase I	Asterina amurensis
24.2	-	purified isozyme alpha-GalNAcase II	Asterina amurensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O	i.e. Forssman hapten glycolipid	Homo sapiens	alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide	-	?
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O	i.e. Forssman hapten glycolipid, production of globoside by both isozymes	Asterina amurensis	alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide	-	?
additional information	the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins	Homo sapiens	?	-	?
additional information	the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins	Asterina amurensis	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 46000, native enzyme, SDS-PAGE	Homo sapiens
monomer	1 * 43000, isozyme alpha-GalNAcase II, SDS-PAGE	Asterina amurensis
polymer	x * 47000, isozyme alpha-GalNAcase I, SDS-PAGE	Asterina amurensis

Synonyms

Synonyms	Comment	Organism
alpha-GalNAcase	-	Homo sapiens
alpha-GalNAcase I	-	Asterina amurensis
alpha-GalNAcase II	-	Asterina amurensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens
37	-	assay at	Asterina amurensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	pH 4.0,m 60 min, purified isozyme alpha-GalNAcase I shows 50% remaining activity, purified isozyme alpha-GalNAcase II 92%	Asterina amurensis
37	-	purified enzyme, 60 min, pH 4.0, 98% activity remaining	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3	-	isozyme alpha-GalNAcase II	Asterina amurensis
3.5	-	isozyme alpha-GalNAcase I	Asterina amurensis
4.2	-	-	Homo sapiens

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2.3	6.5	purified isozyme alpha-GalNAcase II, stable at 4°C	Asterina amurensis
2.7	4.5	purified isozyme alpha-GalNAcase I, stable at 4°C	Asterina amurensis
3.5	8.5	purified enzyme, stable at 4°C	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Asterina amurensis	purified isozyme alpha-GalNAcase I, isoelectric focusing	-	4.4
Homo sapiens	purified enzyme, isoelectric focusing	-	4.8
Asterina amurensis	purified isozyme alpha-GalNAcase II	-	7.1