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Literature summary for 3.2.1.40 extracted from

  • Cui, Z.; Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
    Crystallization and preliminary crystallographic analysis of the family GH78 alpha-L-rhamnosidase RhaB from Bacillus sp. GL1 (2006), Acta Crystallogr. Sect. F, 62, 646-648.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834-DE3 containing pET3a-RhaB plasmid Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
native and selenomethionine-derivaties, crystallized at 293 K, hanging-drop vapour diffusion with PEG 8000 as a precipitant, data collection and statistics indicated Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gellan + H2O Bacillus sp. (in: Bacteria) hydrolytic reaction, release of rhamnose from the disaccharide alpha-L-rhamnose + ?
-
 ?
gellan + H2O Bacillus sp. (in: Bacteria) GL1 hydrolytic reaction, release of rhamnose from the disaccharide alpha-L-rhamnose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q93RE7 rhamnosidase B, RhaB
-
Bacillus sp. (in: Bacteria) GL1 Q93RE7 rhamnosidase B, RhaB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, gel filtration, SDS-PAGE Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay in a reaction system containing 0.4 mM p-nitrophenyl-alpha-L-rhamnopyranoside as a substrate and 50 mM potassium phosphate buffer, resultant p-nitrophenol concentration determined spectrophotometrically Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
4-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) GL1 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
gellan + H2O hydrolytic reaction, release of rhamnose from the disaccharide Bacillus sp. (in: Bacteria) alpha-L-rhamnose + ?
-
 ?
gellan + H2O release of rhamnose from the substrate, hydrolytic reaction Bacillus sp. (in: Bacteria) alpha-L-rhamnose + ?
-
 ?
gellan + H2O hydrolytic reaction, release of rhamnose from the disaccharide Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose + ?
-
 ?
gellan + H2O release of rhamnose from the substrate, hydrolytic reaction Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose + ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-L-rhamnosidase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bacillus sp. (in: Bacteria)