Literature summary for 3.2.1.40 extracted from

Park, S.; Kim, J.; Kim, D.
Purification and characterization of quercitrin-hydrolyzing alpha-L-rhamnosidase from Fusobacterium K-60, a human intestinal bacterium (2005), J. Microbiol. Biotechnol., 15, 519-524.

No PubMed abstract available

Search for more literature for 3.2.1.40

Inhibitors

Inhibitors	Comment	Organism	Structure
4-Chloromercuriphenylsulfonate	strong inhibition	Fusobacterium sp.
Cu2+	strong inhibition	Fusobacterium sp.
L-rhamnose	strong product inhibition	Fusobacterium sp.
Mn2+	strong inhibition	Fusobacterium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.057	-	4-nitrophenyl alpha-L-rhamnopyranoside	pH 5.5-7.0	Fusobacterium sp.
0.077	-	quercitrin	pH 5.5-7.0	Fusobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	-	4 * 41000, SDS-PAGE	Fusobacterium sp.
170000	-	gel filtration	Fusobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
quercitrin + H2O	Fusobacterium sp.	-	quercetin + L-rhamnose	-	?
quercitrin + H2O	Fusobacterium sp. K-60	-	quercetin + L-rhamnose	-	?

Organism

Organism	UniProt	Comment	Textmining
Fusobacterium sp.	-	isolated from human intestine	-
Fusobacterium sp. K-60	-	isolated from human intestine	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity	Fusobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.89	-	purified enzyme	Fusobacterium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O	-	Fusobacterium sp.	4-nitrophenol + alpha-L-rhamnose	-	?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O	-	Fusobacterium sp. K-60	4-nitrophenol + alpha-L-rhamnose	-	?
quercitrin + H2O	-	Fusobacterium sp.	quercetin + L-rhamnose	-	?
quercitrin + H2O	-	Fusobacterium sp. K-60	quercetin + L-rhamnose	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 41000, SDS-PAGE	Fusobacterium sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	7	-	Fusobacterium sp.

pI Value

Organism	Comment	pI Value Maximum	pI Value
Fusobacterium sp.	-	-	5.2

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Release 2023.1 (January 2023)