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Literature summary for 3.2.1.31 extracted from
- Development of a thermostable beta-glucuronidase-based reporter system for monitoring gene expression in hyperthermophiles (2012), Biotechnol. Bioeng., 109, 1881-1886.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | Sulfolobus gene can be used as reporter in any thermophilic microorganism lacking an endogenous beta-glucuronidase activity | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli. Although Escherichia coli possesses an endogenous beta-glucuronidase activity, a short treatment at 80°C of the crude extract causes the precipitation of most Escherichia coli protein, resulting in an enrichment of the Sulfolobus beta-glucuronidase | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
4-methylumbelliferyl beta-D-glucuronide | pH 6.5, 75°C | Saccharolobus solfataricus |  |
| 0.58 | - |
4-methylumbelliferyl beta-D-glucuronide | pH 6.5, 65°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66800 | - |
- |
Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97UI1 | - |
- |
| Saccharolobus solfataricus P2 | Q97UI1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-glucuronide + H2O | - |
Saccharolobus solfataricus | 4-methylumbelliferone + D-glucuronic acid | - |
? | |
| 4-methylumbelliferyl beta-D-glucuronide + H2O | - |
Saccharolobus solfataricus P2 | 4-methylumbelliferone + D-glucuronic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GusB | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | - |
Saccharolobus solfataricus |
| 75 | - |
assay at | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 100 | 37°C: about 40% of maximal activity, 100°C: about 50% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
4.5 d, 1.1% loss of activity | Saccharolobus solfataricus |
| 70 | - |
10 h, enzyme retains full activity | Saccharolobus solfataricus |
| 80 | - |
10 h, enzyme retains full activity | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | highly active in the pH-range 4.5-6.5 | Saccharolobus solfataricus |
| 6.5 | - |
assay at | Saccharolobus solfataricus |
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