Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | - |
endophytic fungus EF6 | |
DTT | - |
endophytic fungus EF6 | |
EDTA | - |
endophytic fungus EF6 | |
glycerol | - |
endophytic fungus EF6 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
endophytic fungus EF6 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
endophytic fungus EF6 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | endophytic fungus EF6 | |
Co2+ | activates | endophytic fungus EF6 | |
Mg2+ | activates | endophytic fungus EF6 | |
Mn2+ | activates | endophytic fungus EF6 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | endophytic fungus EF6 |
62031 | - |
1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry | endophytic fungus EF6 |
62200 | - |
1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry | endophytic fungus EF6 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
endophytic fungus EF6 | - |
isolated from Thai medicinal plants | - |
Purification (Comment) | Organism |
---|---|
native enzyme 14.49fold from culture medium by ammonium sulfate fractionation, anion exchange chromatography and gel filtration | endophytic fungus EF6 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
758 | - |
purified enzyme, substrate is soluble starch, pH 6.0, 50°C | endophytic fungus EF6 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | soluble starch is the best substrate, various raw starches, i.e. soluble starch, corn, tapioca, wheat, rice, sticky rice starch, are used as substrates | endophytic fungus EF6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry | endophytic fungus EF6 |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
endophytic fungus EF6 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
endophytic fungus EF6 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | activity range, profile, overview | endophytic fungus EF6 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, 1 h, stable up to, rapid decrease in activity above | endophytic fungus EF6 |
55 | - |
purified enzyme, 1 h, 45% activity remaining | endophytic fungus EF6 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
endophytic fungus EF6 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity range, profile, overview | endophytic fungus EF6 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified enzyme, 1 h, 20% activity remaining | endophytic fungus EF6 |
4 | 7 | purified enzyme, 1 h, 50°C, stable | endophytic fungus EF6 |
9 | - |
purified enzyme, 1 h, 40% activity remaining | endophytic fungus EF6 |