Application | Comment | Organism |
---|---|---|
industry | glucoamylase is used industrially to catalyze the hydrolysis of alpha-1,4-glycosidic bonds to release beta-D-glucose from the nonreducing ends of starch or oligosaccharides | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis of wild-type and mutant enzymes, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain C468 using vector YEpPM18 | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
D20C/A27C/S30P/G137A | site-directed mutagenesis, the mutant, designated THS8, is highly thermotolerant with increased stability at 80°C compared to the wild-type enzyme | Aspergillus niger |
H391Y | random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme | Aspergillus niger |
additional information | random mutagenesis and mutant screening by plate thermostability assay for increased thermotolerance at 65-80°C, overview | Aspergillus niger |
T290A | random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme | Aspergillus niger |
T62A | random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
maltose | pH 4.5, 35°C, recombinant mutant T62A | Aspergillus niger | |
0.78 | - |
maltose | pH 4.5, 35°C, recombinant mutant T290A | Aspergillus niger | |
0.82 | - |
maltose | pH 4.5, 35°C, recombinant mutant H391Y | Aspergillus niger | |
0.83 | - |
maltose | pH 4.5, 35°C, recombinant wild-type enzyme | Aspergillus niger | |
0.88 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y | Aspergillus niger | |
0.89 | - |
maltose | pH 4.5, 35°C, recombinant mutant S119P | Aspergillus niger | |
1 | - |
maltose | pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A | Aspergillus niger | |
1.16 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/T290A/H391Y | Aspergillus niger | |
1.19 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/H391Y | Aspergillus niger | |
1.35 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/T290A | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Aspergillus niger | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.3 | - |
recombinant mutant S30P/T62A/H391Y | Aspergillus niger |
13.6 | - |
recombinant mutant T62A | Aspergillus niger |
14 | - |
recombinant mutant T290A | Aspergillus niger |
14.2 | - |
recombinant wild-type enzyme | Aspergillus niger |
16.8 | - |
recombinant mutant S119P | Aspergillus niger |
16.9 | - |
recombinant mutant D20C/A27C/S30P/G137A | Aspergillus niger |
17.7 | - |
recombinant mutant H391Y | Aspergillus niger |
17.9 | - |
recombinant mutant S30P/T290A | Aspergillus niger |
18.1 | - |
recombinant mutant S30P/T290A/H391Y | Aspergillus niger |
19.5 | - |
recombinant mutant S30P/H391Y | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Aspergillus niger | beta-D-glucose + D-glucose | - |
? | |
starch + H2O | - |
Aspergillus niger | beta-D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | irreversible thermoinactivation kinetic analysis of recombinant wild-type and mutant enzymes, overview | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | 0.55 | maltose | pH 4.5, 35°C, recombinant mutant T290A | Aspergillus niger | |
0.031 | 0.51 | maltose | pH 4.5, 35°C, recombinant wild-type enzyme | Aspergillus niger | |
0.04 | 1.97 | maltose | pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y | Aspergillus niger | |
0.04 | 1.97 | maltose | pH 4.5, 35°C, recombinant mutant T62A | Aspergillus niger | |
0.052 | 2.1 | maltose | pH 4.5, 35°C, recombinant mutant S30P/H391Y | Aspergillus niger | |
0.057 | 0.65 | maltose | pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A | Aspergillus niger | |
0.5 | - |
maltose | pH 4.5, 35°C, recombinant mutant S119P | Aspergillus niger | |
7.35 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/T290A | Aspergillus niger | |
7.46 | - |
maltose | pH 4.5, 35°C, recombinant mutant T290A | Aspergillus niger | |
7.55 | - |
maltose | pH 4.5, 35°C, recombinant wild-type enzyme | Aspergillus niger | |
8.99 | - |
maltose | pH 4.5, 35°C, recombinant mutant T62A | Aspergillus niger | |
9.3 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/T290A/H391Y | Aspergillus niger | |
9.42 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y | Aspergillus niger | |
10.13 | - |
maltose | pH 4.5, 35°C, recombinant mutant S30P/H391Y | Aspergillus niger | |
10.5 | - |
maltose | pH 4.5, 35°C, recombinant mutant H391Y | Aspergillus niger | |
11.32 | - |
maltose | pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A | Aspergillus niger | |
13.31 | - |
maltose | pH 4.5, 35°C, recombinant mutant S119P | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | 4.5 | assay at | Aspergillus niger |