Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.3 extracted from

  • Michelin, M.; Ruller, R.; Ward, R.J.; Moraes, L.A.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.d.e.L.
    Purification and biochemical characterization of a thermostable extracellular glucoamylase produced by the thermotolerant fungus Paecilomyces variotii (2008), J. Ind. Microbiol. Biotechnol., 35, 17-25.
    View publication on PubMed
Search for more literature for 3.2.1.3

Inhibitors

Inhibitors Comment Organism Structure
Al3+ at 5-10 mM Paecilomyces variotii
Cu2+ at 1-10 mM Paecilomyces variotii
Fe3+
-
 Paecilomyces variotii
Pb2+
-
 Paecilomyces variotii
Zn2+ at 1-10 mM Paecilomyces variotii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 glycogen pH 5.0, 55°C Paecilomyces variotii
2
-
 amylopectin pH 5.0, 55°C Paecilomyces variotii
2.5
-
 amylose pH 5.0, 55°C Paecilomyces variotii
3.8 4.1 starch pH 5.0, 55°C Paecilomyces variotii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paecilomyces variotii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Paecilomyces variotii
additional information only slight effects by Co2+, Ca2+, Ba2+, Mg2+ and Hg2+, and oby other reagents, such as NaCl, NaBr, EDTA, KH2PO4, NaH2PO4, NH4F, NH4Cl, and KCl, overview Paecilomyces variotii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86500
-
 x * 86500, SDS-PAGE Paecilomyces variotii

Organism

Organism UniProt Comment Textmining
Paecilomyces variotii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 47.9fold by anion exchange chromatography and gel filtration Paecilomyces variotii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
465
-
 purified enzyme, substrate starch Paecilomyces variotii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Paecilomyces variotii ?
-
 ?
amylose + H2O low activity Paecilomyces variotii ?
-
 ?
D-malto-oligosaccharides + H2O low activity Paecilomyces variotii ?
-
 ?
glycogen + H2O
-
 Paecilomyces variotii ?
-
 ?
additional information no activity with sucrose, 4-nitrophenyl-beta-D-maltoside, methyl-beta-D-glucopyranoside, pullulan, alpha-cyclodextrin, beta-cyclodextrin, and trehalose Paecilomyces variotii ?
-
 ?
starch + H2O
-
 Paecilomyces variotii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 86500, SDS-PAGE Paecilomyces variotii
More peptide sequencing by mass spectrometry Paecilomyces variotii

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Paecilomyces variotii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Paecilomyces variotii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 temperature profile Paecilomyces variotii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme in absence of substrate, 1 h, stable Paecilomyces variotii
55
-
 purified enzyme in absence of substrate, 1 h, stable Paecilomyces variotii
60
-
 purified enzyme in absence of substrate, half-life is 45 min Paecilomyces variotii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 starch pH 5.0, 55°C Paecilomyces variotii
15.5
-
 amylose pH 5.0, 55°C Paecilomyces variotii
22.1
-
 glycogen pH 5.0, 55°C Paecilomyces variotii
34.1
-
 amylopectin pH 5.0, 55°C Paecilomyces variotii
36.6 41.7 starch pH 5.0, 55°C Paecilomyces variotii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Paecilomyces variotii

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH profile Paecilomyces variotii