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Literature summary for 3.2.1.3 extracted from

  • Riaz, M.; Perveen, R.; Javed, M.R.; Nadeem, H.; Rashid, M.H.
    Kinetic and thermodynamic properties of novel glucoamylase from Humicola sp. (2007), Enzyme Microb. Technol., 41, 558-564.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and thermodynamics Humicola sp.
0.26
-
soluble starch pH 4.7, 55°C Humicola sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Humicola sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72800
-
gel filtration Humicola sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Humicola sp.
-
D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Humicola sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 39fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography Humicola sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture submerged growth condition Humicola sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.44
-
cell culture Humicola sp.
406.9
-
purified native enzyme Humicola sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
Humicola sp. beta-D-glucose + ?
-
?
soluble starch + H2O
-
Humicola sp. beta-D-glucose + soluble starch
-
?
starch + H2O
-
Humicola sp. D-glucose + ?
-
?
starch + H2O soluble starch Humicola sp. D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 72800, SDS-PAGE Humicola sp.

Synonyms

Synonyms Comment Organism
glucoamylase
-
Humicola sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Humicola sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69
-
soluble starch pH 4.7, 55°C Humicola sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
-
Humicola sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.9
-
Humicola sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 5.9 purified native extracellular enzyme, stable Humicola sp.