KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics | Humicola sp. | |
0.26 | - |
soluble starch | pH 4.7, 55°C | Humicola sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Humicola sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72800 | - |
gel filtration | Humicola sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Humicola sp. | - |
D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 39fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography | Humicola sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | submerged growth condition | Humicola sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.44 | - |
cell culture | Humicola sp. |
406.9 | - |
purified native enzyme | Humicola sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Humicola sp. | beta-D-glucose + ? | - |
? | |
soluble starch + H2O | - |
Humicola sp. | beta-D-glucose + soluble starch | - |
? | |
starch + H2O | - |
Humicola sp. | D-glucose + ? | - |
? | |
starch + H2O | soluble starch | Humicola sp. | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72800, SDS-PAGE | Humicola sp. |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Humicola sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Humicola sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69 | - |
soluble starch | pH 4.7, 55°C | Humicola sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
- |
Humicola sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.9 | - |
Humicola sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.9 | purified native extracellular enzyme, stable | Humicola sp. |